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Quantitation of trace polyfunctional thiols in wine by liquid chromatography quadrupole Orbitrap high-resolution mass spectrometry in parallel reaction monitoring

dc.rights.licenseopenen_US
hal.structure.identifierUnité de Recherche Œnologie [Villenave d'Ornon] [OENO]
dc.contributor.authorCHEN, Liang
hal.structure.identifierUnité de Recherche Œnologie [Villenave d'Ornon] [OENO]
dc.contributor.authorDE LONGHI, Emilio
hal.structure.identifierUnité de Recherche Œnologie [Villenave d'Ornon] [OENO]
dc.contributor.authorGAMMACURTA, Marine
hal.structure.identifierUnité de Recherche Œnologie [Villenave d'Ornon] [OENO]
dc.contributor.authorMARCHAL, Axel
IDREF: 150146477
hal.structure.identifierUnité de Recherche Œnologie [Villenave d'Ornon] [OENO]
dc.contributor.authorDARRIET, Philippe
IDREF: 077788834
dc.date.accessioned2024-09-19T12:08:48Z
dc.date.available2024-09-19T12:08:48Z
dc.date.issued2024-09-07
dc.identifier.issn0021-9673en_US
dc.identifier.urioai:crossref.org:10.1016/j.chroma.2024.465360
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/201690
dc.description.abstractEnPolyfunctional thiols are key contributors to wine aroma due to their extremely low odor thresholds, and their quantitative analysis remains challenging as a result of their ultra-trace concentrations and high reactivity. This work presents the first method based on Orbitrap highresolution mass spectrometry (HRMS) in parallel reaction monitoring (PRM) mode for quantifying thiols at ng/L levels in wine. Thiols in wine were derivatized using 4,4'-dithiodipyridine and isolated by liquid-liquid extraction. This protocol allowed rapid sample preparation with minimum labor input and low consumable expenses. Instrumental analysis was conducted using ultra-highperformance liquid chromatography (UHPLC) coupled to quadrupole Orbitrap HRMS in PRM mode. Twenty thiol analytes, including literature-known, recently identified, and novel thiols were selected and validated by the optimized method in three wine matrices. The overall analytical performances demonstrated by this method were equivalent, and in most cases, greater than many previously developed GC–MS or LC–MS methods. The validated method was applied to analyze a selection of wines in which 12 thiols were quantified.
dc.language.isoENen_US
dc.sourcecrossref
dc.subject.enWine
dc.subject.enThiols
dc.subject.enQuantitation
dc.subject.enOrbitrap
dc.subject.enParallel reaction monitoring
dc.title.enQuantitation of trace polyfunctional thiols in wine by liquid chromatography quadrupole Orbitrap high-resolution mass spectrometry in parallel reaction monitoring
dc.typeArticle de revueen_US
dc.identifier.doi10.1016/j.chroma.2024.465360en_US
dc.subject.halSciences du Vivant [q-bio]/Biologie végétaleen_US
bordeaux.journalJournal of Chromatography Aen_US
bordeaux.page465360en_US
bordeaux.hal.laboratoriesUnité de Recherche Œnologie [Villenave d'Ornon] [OENO]en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionBordeaux INPen_US
bordeaux.institutionINRAEen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressouien_US
bordeaux.import.sourcedissemin
hal.identifierhal-04702555
hal.version1
hal.date.transferred2024-09-19T12:08:51Z
hal.popularnonen_US
hal.audienceInternationaleen_US
hal.exporttrue
workflow.import.sourcedissemin
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Journal%20of%20Chromatography%20A&rft.date=2024-09-07&rft.spage=465360&rft.epage=465360&rft.eissn=0021-9673&rft.issn=0021-9673&rft.au=CHEN,%20Liang&DE%20LONGHI,%20Emilio&GAMMACURTA,%20Marine&MARCHAL,%20Axel&DARRIET,%20Philippe&rft.genre=article


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