SANTA, Pauline; ROUBERTIE, Anaïs; LOIZON, Severine; GARREAU, Anne; FERRIERE, Amandine; DULUC, Dorothee; SISIRAK, Vanja

doi:10.1007/978-1-0716-2938-3_13

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SANTA, Pauline
Immunology from Concept and Experiments to Translation = Immunologie Conceptuelle, Expérimentale et Translationnelle [ImmunoConcept]

ROUBERTIE, Anaïs
Immunology from Concept and Experiments to Translation = Immunologie Conceptuelle, Expérimentale et Translationnelle [ImmunoConcept]

LOIZON, Severine
Immunology from Concept and Experiments to Translation = Immunologie Conceptuelle, Expérimentale et Translationnelle [ImmunoConcept]

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Dendritric cells. 2023-03, vol. 2618, p. 173-186

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Dendritic cells (DCs) are antigen-presenting cells (APCs) that shape innate and adaptive immunity. There are multiple subsets of DCs distinguished according to their phenotype and functional specialization. DCs are present in lymphoid organs and across multiple tissues. However, their frequency and numbers at these sites are very low making their functional study difficult. Multiple protocols have been developed to generate DCs in vitro from bone marrow progenitors, but they do not fully recapitulate DC complexity found in vivo. Therefore, directly amplifying endogenous DCs in vivo appears as an option to overcome this specific caveat. In this chapter, we describe a protocol to amplify murine DCs in vivo by the injection of a B16 melanoma cell line expressing the trophic factor FMS-like tyrosine kinase 3 ligand (Flt3L). We have also compared two methods of magnetic sorting of amplified DCs, both giving high yields of total murine DCs, but different representation of the main DC subsets found in vivo. © 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.< Réduire

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Conventional dendritic cells

DC enrichment

Flt3L

Plasmacytoid dendritic cells

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Enrichment of Large Numbers of Splenic Mouse Dendritic Cells After Injection of Flt3L-Producing Tumor Cells

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