BOULANGE-LECOMTE, Céline; ROCHER, Béatrice; CAILLEAUD, Kevin; COSETTE, Pascal; LEGRAND, Eléna; DEVREKER, David; BUDZINSKI, Hélène; SOUISSI, Sami; FORGET-LERAY, Joëlle

doi:10.1002/etc.3343

BOULANGE-LECOMTE, Céline
Laboratoire d'Ecotoxicologie - Milieux Aquatiques [LEMA]

ROCHER, Béatrice
Stress Environnementaux et BIOsurveillance des milieux aquatiques [SEBIO]

CAILLEAUD, Kevin
Stress Environnementaux et BIOsurveillance des milieux aquatiques [SEBIO]
Environnements et Paléoenvironnements OCéaniques [EPOC]
Laboratoire d’Océanologie et de Géosciences (LOG) - UMR 8187 [LOG]

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Environmental Toxicology and Chemistry. 2016, vol. 35, n° 7, p. 1860 - 1871

Résumé en anglais

Proteomics was used in the calanoid copepod Eurytemora affinis for screening of protein expression modifications induced by organic contaminants. The copepods were exposed in a continuous flow-through system for 86 h to environmentally relevant concentrations of contaminants representative of the pollution in the Seine Estuary (Haute-Normandie, France; diuron, 500 ng L−1; alkylphenol mixture, 1000 ng L−1). Proteome analysis of whole-body copepod extracts by 2-dimensional gel electrophoresis revealed that the contaminants induced modifications in protein expression, with the highest quantitative variations occurring after diuron exposure. Specifically, 88 and 41 proteins were differentially expressed after diuron and alkylphenol treatments, respectively. After mass spectrometry analysis, 51 (diuron exposure) and 15 (alkylphenol exposure) proteins were identified. The identified proteins were potentially related to energy metabolism, cell growth, nervous signal conductivity, excitotoxicity, oxidative stress response, and antioxidant defense. The data suggest a massive general disturbance of physiological functions of E. affinis after diuron exposure, whereas alkylphenols induced an alteration of a few targeted physiological functions. The protein expression signatures identified after contaminant exposure deserve further investigation in terms of the development of novel potential biomarkers for water quality assessment< Réduire

Mots clés en anglais

2D Electrophoresis

Biomarkers

Calanoid

Estuary

Organic contaminants

URI

https://oskar-bordeaux.fr/handle/20.500.12278/201383

DOI

http://dx.doi.org/10.1002/etc.3343

Unités de recherche

EPOC : Environnements et Paléoenvironnements Océaniques et Continentaux - UMR 5805

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Differential protein expression in the estuarine copepod Eurytemora affinis after diuron and alkylphenol exposures

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Résumé en anglais

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