PANNETIER, Pauline; CLERANDEAU, Christelle; LE FLOCH, Stéphane; CACHOT, Jérôme; MORIN, Benedicte

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PANNETIER, Pauline

CLERANDEAU, Christelle
Environnements et Paléoenvironnements OCéaniques [EPOC]

LE FLOCH, Stéphane

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Article de revue

Ce document a été publié dans

Environmental Science and Pollution Research. 2024-07-30

Résumé en anglais

Fish are currently used models for the toxicity assessment of chemicals, including polycyclic aromatic hydrocarbons (PAHs). Alternative methods including fish cell lines are currently used to provide fast and reliable results on the toxic properties of chemicals while respecting ethical concerns about animal testing. The Rainbow trout liver cell line RTLW1 was used to analyze the effects of two water-accommodated fractions from two crude oils: Arabian Light crude oil (LO) and refined oil from Erika (HO). Several toxicity endpoints were assessed in this study, including cytotoxicity, EROD activity, DNA damage (comet and micronucleus assays), and ROS production. RTL-W1 cells were exposed for 24 h at two or three dilutions of WAF at 1000 µg/L (0.1% (1 μg/L), 1% (10 μg/L), and 10% (100 μg/L)) for cytotoxicity and EROD activity and 1% and 10% for ROS production and genotoxicity). Exposure of RTL-W1 cells to LO WAF induced a significant increase of EROD activity and ROS production and altered DNA integrity as revealed by both the comet assay and the micronucleus test for 10 µg/L of LO. On the other hand, HO WAF exhibited limited toxic effects except for an EROD induction for 1% WAF dilution. These results confirmed the usefulness of RTL-W1 cells for in vitro toxicological assessment of chemical mixtures.< Réduire

URI

https://oskar-bordeaux.fr/handle/20.500.12278/201255

Unités de recherche

EPOC : Environnements et Paléoenvironnements Océaniques et Continentaux - UMR 5805