SERRA, Helene; BRION, François; PORCHER, Jean-Marc; BUDZINSKI, Hélène; AIT-AISSA, Selim

doi:10.3390/ijms19041175

Voir/Ouvrir

2018-123.pdf (9.334Mo)

Métadonnées

Afficher la notice complète

Licence d’utilisation du document

SERRA, Helene
Laboratoire de Physico et Toxico-Chimie des systèmes naturels [LPTC]
Environnements et Paléoenvironnements OCéaniques [EPOC]
Institut National de l'Environnement Industriel et des Risques [INERIS]

BRION, François
Institut National de l'Environnement Industriel et des Risques [INERIS]

PORCHER, Jean-Marc
Institut National de l'Environnement Industriel et des Risques [INERIS]

Langue

Article de revue

Ce document a été publié dans

International Journal of Molecular Sciences. 2018, vol. 19, p. art. 1175

Résumé en anglais

Triclosan (TCS), an antimicrobial agent widely found in the aquatic environment, is suspected to act as an endocrine disrupting compound, however mechanistic information is lacking in regards to aquatic species. This study assessed the ability of TCS to interfere with estrogen receptor (ER) transcriptional activity, in zebrafish-specific in vitro and in vivo reporter gene assays. We report that TCS exhibits a lack of either agonistic or antagonistic effects on a panel of ER-expressing zebrafish (ZELH-zfER alpha and -zfER beta) and human (MELN) cell lines. At the organism level, TCS at concentrations of up to 0.3 mu M had no effect on ER-regulated brain aromatase gene expression in transgenic cyp19a1b-GFP zebrafish embryos. At a concentration of 1 mu M, TCS interfered with the E2 response in an ambivalent manner by potentializing a low E2 response (0.625 nM), but decreasing a high E2 response (10 nM). Altogether, our study suggests that while modulation of ER-regulated genes by TCS may occur in zebrafish, it does so irrespective of a direct binding and activation of zfERs.< Réduire

Mots clés en anglais

In vitro

In vivo

Estrogen receptor

Zebrafish

Triclosan

Brain aromatase