Utilisation des Rapid Light Curves pour l'évaluation de la toxicité des pesticides sur les biofilms

KIM TIAM, Sandra; LAVIALE, M.; FEURTET-MAZEL, Agnes; JAN, G.; GONZALEZ, Patrice; MAZZELLA, Nicolas; MORIN, Soizic

doi:10.1016/j.aquatox.2015.05.001

Métadonnées

Afficher la notice complète

Licence d’utilisation du document

KIM TIAM, Sandra
Environnements et Paléoenvironnements OCéaniques [EPOC]

LAVIALE, M.
UNIVERSIDADE DE AVEIRO DEPARTAMENTO DE BIOLOGIA AND CESAM AVEIRO PRT
Centre National de la Recherche Scientifique [CNRS]

FEURTET-MAZEL, Agnes
Environnements et Paléoenvironnements OCéaniques [EPOC]

Langue

Article de revue

Ce document a été publié dans

Aquatic Toxicology. 2015-08, vol. 165, p. 160-171

Résumé en anglais

The use of Rapid light curves (RLCs) as a toxicity endpoint for river biofilms was examined in this studyand compared to "classical fluorescence parameters" i.e. minimal fluorescence (F0), optimal and effectivequantum yields of photosystem II (Fv/Fmand PSII). Measurements were performed after exposure tofive concentrations of diuron (from 0.3 to 33.4 g L-1), its main degradation product (DCPMU) (from1.0 to 1014 g L-1) and norflurazon (from 0.6 to 585 g L-1) with the lowest exposure concentrationscorresponding to levels regularly encountered in chronically contaminated sites. Biofilm responses wereevaluated after 1, 5, 7 and 14 days of exposure to the different toxicants.Overall, the responses of both "classical fluorescence parameters" and RLC endpoints were highly timedependent and related to the mode of action of the different compounds. Interestingly, parameters cal-culated from RLCs (ETRmaxand Ik) were useful early markers of pesticide exposure since they revealedsignificant effects of all the tested toxicants from the first day of exposure. In comparison, classicalfluorescence endpoints (F0and Fv/Fm) measured at day 1 were only affected in the DCPMU treatment.Our results demonstrated the interest of RLCs as early markers of toxicant exposure particularly whenworking with toxicants with less specific mode of action than PSII inhibitors.< Réduire

Métadonnées

Partager cette publication !

Licence d’utilisation du document