COELHO, Christian; PAROT, Jérémie; GONSIOR, Michael; NIKOLANTONAKI, Maria; SCHMITT-KOPPLIN, Philippe; PARLANTI, Edith; GOUGEON, Régis D.

doi:10.1007/s00216-017-0221-1

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COELHO, Christian
Procédés Alimentaires et Microbiologiques [Dijon] [PAM]

PAROT, Jérémie
Laboratoire de Physico et Toxico-Chimie des systèmes naturels [LPTC]
Environnements et Paléoenvironnements OCéaniques [EPOC]

GONSIOR, Michael
Chesapeake Biological Laboratory [CBL]

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Analytical and Bioanalytical Chemistry. 2017-04, vol. 409, n° 10, p. 2757 - 2766

Résumé en anglais

wo analytical separation methods-size-exclusion chromatography and asymmetrical flow field-flow fractionation- were implemented to evaluate the integrity of the colloidal composition of Chardonnay white wine and the impact of pressing and fermentations on the final macromolecular composition. Wine chromophoric colloidal matter, representing UV-visible-absorbing wine macromolecules, was evaluated by optical and structural measurements combined with the description of elution profiles obtained by both separative techniques. The objective of this study was to apply these two types of fractionation on a typical Chardonnay white wine produced in Burgundy and to evaluate how each of them impacted the determination of the macromolecular chromophoric content of wine. UV-visible and fluorescence measurements of collected fractions were successfully applied. An additional proteomic study revealed that grape and microorganism proteins largely impacted the composition of chromophoric colloidal matter of Chardonnay wines. Asymmetrical flow field-flow fractionation appeared to be more reliable and less invasive with respect to the native chemical environment of chromophoric wine macromolecules, and hence is recommended as a tool to fractionate chromophoric colloidal matter in white wines.< Réduire

Mots clés en anglais

Mannoproteins

polysaccharides

Yeast

Molecular-weight

Humic substances

Maceration enzymes

Red wines

Haze-forming proteins

Dissolved organic-matter

Asymmetrical flow field-flow fractionation

Size-exclusion chromatography

Wine proteins

White wine

Chromophoric colloidal matter

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Asymmetrical flow field-flow fractionation of white wine chromophoric colloidal matter

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Mots clés en anglais

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