Oyster transcriptome response to Alexandrium exposure is related to saxitoxin load and characterized by disrupted digestion, energy balance, and calcium and sodium signaling
MAT, Audrey
Laboratoire des Sciences de l'Environnement Marin (LEMAR) [LEMAR]
Institut Français de Recherche pour l'Exploitation de la Mer [IFREMER]
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Laboratoire des Sciences de l'Environnement Marin (LEMAR) [LEMAR]
Institut Français de Recherche pour l'Exploitation de la Mer [IFREMER]
MAT, Audrey
Laboratoire des Sciences de l'Environnement Marin (LEMAR) [LEMAR]
Institut Français de Recherche pour l'Exploitation de la Mer [IFREMER]
Laboratoire des Sciences de l'Environnement Marin (LEMAR) [LEMAR]
Institut Français de Recherche pour l'Exploitation de la Mer [IFREMER]
CHALOPIN, Morgane
Laboratoire des Sciences de l'Environnement Marin (LEMAR) [LEMAR]
Institut Français de Recherche pour l'Exploitation de la Mer [IFREMER]
Laboratoire des Sciences de l'Environnement Marin (LEMAR) [LEMAR]
Institut Français de Recherche pour l'Exploitation de la Mer [IFREMER]
HUVET, Arnaud
Laboratoire des Sciences de l'Environnement Marin (LEMAR) [LEMAR]
Institut Français de Recherche pour l'Exploitation de la Mer [IFREMER]
< Réduire
Laboratoire des Sciences de l'Environnement Marin (LEMAR) [LEMAR]
Institut Français de Recherche pour l'Exploitation de la Mer [IFREMER]
Langue
EN
Article de revue
Ce document a été publié dans
Aquatic Toxicology. 2018-06, vol. 199, p. 127-137
Résumé en anglais
Harmful Algal Blooms are worldwide occurrences that can cause poisoning in human seafood consumers as well as mortality and sublethal effets in wildlife, propagating economic losses. One of the most widespread toxigenic ...Lire la suite >
Harmful Algal Blooms are worldwide occurrences that can cause poisoning in human seafood consumers as well as mortality and sublethal effets in wildlife, propagating economic losses. One of the most widespread toxigenic microalgal taxa is the dinoflagellate Genus Alexandrium, that includes species producing neurotoxins referred to as PST (Paralytic Shellfish Toxins). Blooms cause shellfish harvest restrictions to protect human consumers from accumulated toxins. Large inter-individual variability in toxin load within an exposed bivalve population complicates monitoring of shellfish toxicity for ecology and human health regulation. To decipher the physiological pathways involved in the bivalve response to PST, we explored the whole transcriptome of the digestive gland of the Pacific oyster Crassostrea gigas fed experimentally with a toxic Alexandrium minutum culture. The largest differences in transcript abundance were between oysters with contrasting toxin loads (1098 transcripts), rather than between exposed and non-exposed oysters (16 transcripts), emphasizing the importance of toxin load in oyster response to toxic dinoflagellates. Additionally, penalized regressions, innovative in this field, modeled accurately toxin load based upon only 70 transcripts. Transcriptomic differences between oysters with contrasting PST burdens revealed a limited suite of metabolic pathways affected, including ion channels, neuromuscular communication, and digestion, all of which are interconnected and linked to sodium and calcium exchanges. Carbohydrate metabolism, unconsidered previously in studies of harmful algal effects on shellfish, was also highlighted, suggesting energy challenge in oysters with high toxin loads. Associations between toxin load, genotype, and mRNA levels were revealed that open new doors for genetic studies identifying genetically-based low toxin accumulation.< Réduire
Mots clés en anglais
Transcriptomic
Ions channels
ACL
Paralytic shellfish toxins
Elastic-net regression
Project ANR
De la caractérisation des déterminants de l'accumulation des toxines paralysantes (PST) chez l'huître (Crassostrea gigas) au risque sanitaire pour l'homme dans son contexte sociétal - ANR-13-CESA-0019