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dc.rights.licenseopenen_US
dc.contributor.authorBEN ALI, Naouar
dc.contributor.authorBENKADDOUR, Rajae
dc.contributor.authorRAHMOUNI, Safaa
dc.contributor.authorHAMDOUN, Ouafaa
dc.contributor.authorBOUSSAOUDI, Ibtissam
dc.contributor.authorHASSOUN, Mustapha
dc.contributor.authorAZAROUAL, Latifa
hal.structure.identifierUnité de Recherche Œnologie [Villenave d'Ornon] [OENO]
dc.contributor.authorBADOC, Alain
dc.contributor.authorMARTIN, Patrick
dc.contributor.authorLAMARTI, Ahmed
dc.date.accessioned2024-04-23T07:59:37Z
dc.date.available2024-04-23T07:59:37Z
dc.date.issued2023
dc.identifier.issn2165-5979en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/199268
dc.description.abstractEnQuercus suber L. is the main woody tree species in the Mediterranean basin. The in vitro regeneration from adult material, through primary somatic embryogenesis, is a well-known process, but the use of secondary somatic embryos for plant regeneration remains a very sparsely studied process. The main objective of this work is to explore the cork oak regeneration potential by using the secondary somatic embryogenesis process. Mainly, in this work, we report the polyamine effect. Explants used consisted on primary mature embryos, derived from leaves rejuvenated by epicormic shoot of the Moroccan Quercus suber. Three different polyamines were added to the basal medium, which was composed by macronutrients of N30K, 30 g/l glucose, and 7 g/l agar. Three polyamines, Putrescine, Spermine, and Spermidine, were added to the basal medium at 0.1, 0.2, 0.3, 0.4, 0.5, and 0.6 mg/l. Explants were tested after 8 weeks. Morphological analysis showed that the medium with 0.4 mg/l Spermidine provided the best result for secondary embryos, which corresponds to a very significant (p < 0.05) increase of 375%. The number of secondary embryos directly formed was 2.70 ± 0.51. Similarly, the optimum concentrations for high number of clusters (0.50 ± 0.11) and embryo clusters (1.43 ± 0.35) were increased by 145% and 158%. The addition of the polyamine also acted on the quality of embryos formed. A very significant (p < 0.05) increase in the size of secondary embryos was observed compared to the medium without polyamines. Spermidine showed the greatest increase (about 38%).
dc.language.isoENen_US
dc.rightsAttribution-NonCommercial 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc/3.0/us/*
dc.subject.enCork oak
dc.subject.enSecondary embryos
dc.subject.enSomatic embryos
dc.subject.enPutrescine
dc.subject.enSpermidine
dc.subject.enSpermine
dc.title.enInfluence of exogenous polyamines on the secondary somatic embryogenesis of cork oak (Quercus suber L.)
dc.typeArticle de revueen_US
dc.identifier.doi10.1080/21655979.2023.2288354en_US
dc.subject.halSciences du Vivant [q-bio]/Biologie végétaleen_US
bordeaux.journalBioengineereden_US
bordeaux.volume14en_US
bordeaux.hal.laboratoriesOenologie - UMR 1366en_US
bordeaux.issue1en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionBordeaux INPen_US
bordeaux.institutionINRAEen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
hal.identifierhal-04555621
hal.version1
hal.date.transferred2024-04-23T07:59:40Z
hal.popularnonen_US
hal.audienceInternationaleen_US
hal.exporttrue
dc.rights.ccCC BY-NCen_US
bordeaux.COinSctx_ver=Z39.88-2004&amp;rft_val_fmt=info:ofi/fmt:kev:mtx:journal&amp;rft.jtitle=Bioengineered&amp;rft.date=2023&amp;rft.volume=14&amp;rft.issue=1&amp;rft.eissn=2165-5979&amp;rft.issn=2165-5979&amp;rft.au=BEN%20ALI,%20Naouar&amp;BENKADDOUR,%20Rajae&amp;RAHMOUNI,%20Safaa&amp;HAMDOUN,%20Ouafaa&amp;BOUSSAOUDI,%20Ibtissam&amp;rft.genre=article


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