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Enhanced Wound Healing Potential of Spirulina platensis Nanophytosomes: Metabolomic Profiling, Molecular Networking, and Modulation of HMGB-1 in an Excisional Wound Rat Model

dc.rights.licenseopenen_US
dc.contributor.authorREFAI, Hanan
dc.contributor.authorEL-GAZAR, Amira A
dc.contributor.authorRAGAB, Ghada M
dc.contributor.authorHASSAN, Doaa H
hal.structure.identifierUnité de Recherche Œnologie [Villenave d'Ornon] [OENO]
dc.contributor.authorAHMED, Omar S
dc.contributor.authorHUSSEIN, Rehab A
dc.contributor.authorSHABANA, Samah
hal.structure.identifierUnité de Recherche Œnologie [Villenave d'Ornon] [OENO]
dc.contributor.authorWAFFO-TÉGUO, Pierre
hal.structure.identifierUnité de Recherche Œnologie [Villenave d'Ornon] [OENO]
hal.structure.identifierPlateforme Bordeaux Metabolome
dc.contributor.authorVALLS, Josep
dc.contributor.authorAL-MOKADDEM, Asmaa K
dc.contributor.authorSELIM, Heba Mohammed Refat M.
dc.contributor.authorYOUSEF, Einas Mohamed
dc.contributor.authorALI, Sahar K
dc.contributor.authorSALMAN, Ahmed
dc.contributor.authorABO-ZALAM, Hagar B
dc.contributor.authorALBASH, Rofida
dc.date.accessioned2024-04-10T12:53:54Z
dc.date.available2024-04-10T12:53:54Z
dc.date.issued2023-02
dc.identifier.issn1660-3397en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/197419
dc.description.abstractEnExcisional wounds are considered one of the most common physical injuries. This study aims to test the effect of a nanophytosomal formulation loaded with a dried hydroalcoholic extract of S. platensis on promoting excisional wound healing. The Spirulina platensis nanophytosomal formulation (SPNP) containing 100 mg PC and 50 mg CH exhibited optimum physicochemical characteristics regarding particle size (598.40 ± 9.68 nm), zeta potential (−19.8 ± 0.49 mV), entrapment efficiency (62.76 ± 1.75%), and Q6h (74.00 ± 1.90%). It was selected to prepare an HPMC gel (SPNP-gel). Through metabolomic profiling of the algal extract, thirteen compounds were identified. Molecular docking of the identified compounds on the active site of the HMGB-1 protein revealed that 12,13-DiHome had the highest docking score of −7.130 kcal/mol. SPNP-gel showed higher wound closure potential and enhanced histopathological alterations as compared to standard (MEBO® ointment) and S. platensis gel in wounded Sprague-Dawley rats. Collectively, NPS promoted the wound healing process by enhancing the autophagy process (LC3B/Beclin-1) and the NRF-2/HO-1antioxidant pathway and halting the inflammatory (TNF-, NF-κB, TlR-4 and VEGF), apoptotic processes (AIF, Caspase-3), and the downregulation of HGMB-1 protein expression. The present study’s findings suggest that the topical application of SPNP-gel possesses a potential therapeutic effect in excisional wound healing, chiefly by downregulating HGMB-1 protein expression. © 2023 by the authors.
dc.language.isoENen_US
dc.rightsAttribution 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/us/*
dc.subject.enblue green algae
dc.subject.enHMGB-1
dc.subject.enmolecular docking
dc.subject.enmolecular networking
dc.subject.enwound healing
dc.title.enEnhanced Wound Healing Potential of Spirulina platensis Nanophytosomes: Metabolomic Profiling, Molecular Networking, and Modulation of HMGB-1 in an Excisional Wound Rat Model
dc.title.alternativeMar. Drugsen_US
dc.typeArticle de revueen_US
dc.identifier.doi10.3390/md21030149en_US
dc.subject.halSciences du Vivant [q-bio]/Biologie végétaleen_US
bordeaux.journalMarine Drugsen_US
bordeaux.page149en_US
bordeaux.volume21en_US
bordeaux.hal.laboratoriesOenologie - UMR 1366en_US
bordeaux.issue3en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionBordeaux INPen_US
bordeaux.institutionINRAEen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
hal.popularnonen_US
hal.audienceInternationaleen_US
hal.exportfalse
dc.rights.ccCC BYen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Marine%20Drugs&rft.date=2023-02&rft.volume=21&rft.issue=3&rft.spage=149&rft.epage=149&rft.eissn=1660-3397&rft.issn=1660-3397&rft.au=REFAI,%20Hanan&EL-GAZAR,%20Amira%20A&RAGAB,%20Ghada%20M&HASSAN,%20Doaa%20H&AHMED,%20Omar%20S&rft.genre=article


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