ZHAO, Hang; IBRAHIMOVA, Vusala; GARANGER, Elisabeth; LECOMMANDOUX, Sebastien

doi:10.1002/anie.202001868

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ZHAO, Hang
Laboratoire de Chimie des Polymères Organiques [LCPO]
Team 3 LCPO : Polymer Self-Assembly & Life Sciences

IBRAHIMOVA, Vusala
Laboratoire de Chimie des Polymères Organiques [LCPO]
Team 3 LCPO : Polymer Self-Assembly & Life Sciences

GARANGER, Elisabeth

Laboratoire de Chimie des Polymères Organiques [LCPO]
Team 3 LCPO : Polymer Self-Assembly & Life Sciences

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Article de revue

Ce document a été publié dans

Angewandte Chemie International Edition. 2020, vol. 59, n° 27, p. 11028-11036

Wiley-VCH Verlag

Résumé en anglais

Elastin‐like polypeptides (ELPs) have been proposed as a simple model of intrinsically disordered proteins (IDPs) which can form membrane‐less organelles via liquid‐liquid phase separation (LLPS) in cellular milieu. Herein, fluorescently labeled ELP is studied in cytomimetic aqueous two‐phase system (ATPS). Droplet‐based protocells are obtained in a microfluidic system, allowing for confinement, temperature changes and statistical analysis. The spatial organization of ELP is observed in such binary ATPS macrocrowders. In addition, owing to switch of conformational states, dynamic formation and distribution of ELP‐rich droplets within the artificial cytoplasm is triggered by temperature. Three‐dimensional structured proteins are concurrently encapsulated along with ELP in synthetic cells and distinct partitioning properties of these proteins and ELP in binary polymeric phases are observed. This underpinning discovery demonstrates that the ability of ELP to coacervate with temperature can be maintained inside intracellular mimetic medium, and the preferential distribution of ELP in macromolecular crowding.< Réduire

Mots clés en anglais

Intrinsically disordered proteins (IDP)

Elastin‐like polypeptides (ELP)

synthetic cell

minimal cell

macromolecular crowding

aqueous two‐phase system (ATPS)

URI

https://oskar-bordeaux.fr/handle/20.500.12278/19698

DOI

http://dx.doi.org/10.1002/anie.202001868

Projet Européen

Temperature-responsive polypeptide nanocarriers for abdominal therapies

Project ANR

IdEx Bordeaux - ANR-10-IDEX-0003-02/10-IDEX-0003

Origine

Importé de hal

Unités de recherche

Laboratoire de Chimie des Polymères Organiques (LCPO) - UMR 5629