CHEN, Mingfu; GRAZON, Chloé; SENSHARMA, Prerana; NGUYEN, Thuy; FENG, Yunpeng; CHERN, Margaret; BAER, R.; VARONGCHAYAKUL, Nitinun; COOK, Katherine; LECOMMANDOUX, Sebastien; KLAPPERICH, Catherine; GALAGAN, James; DENNIS, Allison; GRINSTAFF, Mark

doi:10.1021/acsami.0c13489

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CHEN, Mingfu
Boston University [Boston] [BU]

GRAZON, Chloé
Boston University [Boston] [BU]
Laboratoire de Chimie des Polymères Organiques [LCPO]
Team 3 LCPO : Polymer Self-Assembly & Life Sciences

SENSHARMA, Prerana
Boston University [Boston] [BU]

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Article de revue

Ce document a été publié dans

ACS Applied Materials & Interfaces. 2020, vol. 12, p. doi 10.1021/acsami.0c13489

Washington, D.C. : American Chemical Society

Résumé en anglais

Immobilization of biosensors in or on a functional material is critical for subsequent device development and translation to wearable technology. Here we present the development and assessment of an immobilized quantum dot - transcription factor - nucleic acid complex for progesterone detection as a first step toward such device integration. The sensor is composed of a polyhistidine-tagged transcription factor linked to a quantum dot and a fluorophore-modified cognate DNA, and embedded within a hydrogel as an immobilization matrix. The hydrogel is optically transparent, soft, and flexible as well as traps the quantum dot - transcription factor DNA assembly but allows free passage of the analyte, progesterone. Upon progesterone exposure, DNA dissociates from the quantum dot - transcription factor DNA assembly resulting in an attenuated ratiometric fluorescent output via Förster resonance energy transfer. The sensor performs in a dose-dependent manner with a limit of detection of 55 nM. Repeat analyte measurements are also similarly successful. Our approach combines a systematically characterized hydrogel as an immobilization matrix and a transcription factor - DNA binding as a recognition/ transduction element, offering a promising framework for future biosensor devices based upon allosteric transcription factor.< Réduire

Mots clés en anglais

biosensing

quantum dots

transcription factor

hydrogel

Förster resonance energy transfer

URI

https://oskar-bordeaux.fr/handle/20.500.12278/19568

DOI

http://dx.doi.org/10.1021/acsami.0c13489

Projet Européen

A Wearable Sensor for Hormones Based on a Native Microbial Sensing

Origine

Importé de hal

Unités de recherche

Laboratoire de Chimie des Polymères Organiques (LCPO) - UMR 5629