The physical properties of membranes of hybrid polymer lipid vesicles are so far relatively unknown. Since their discovery a decade ago, many studies have aimed to show their great potential in many fields of application, but so far, few systematic studies have been carried out to decipher the relationship between the molecular characteristics of the components (molar mass, chemical nature, and architecture of the copolymer), the membrane structure and its properties. In this work, we study the association of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and poly(dimethylsiloxane)-b-poly(ethylene oxide) (PDMS-b-PEO) diblock copolymers of different molar masses in giant hybrid vesicles and establish a complete phase diagram of the membrane structure. We also measured the mechanical properties of the giant hybrid unilamellar vesicle (GHUV) through micropipette aspiration at different lipid/polymer compositions. Thanks to a previous work using triblock PEO-b-PDMS-b-PEO copolymers, we were able to reveal the effect of the architecture of the block copolymer on membrane structure and properties. Besides, the association of diblock copolymers PDMS-b-PEO and POPC leads to the formation of hybrid vesicles with unprecedented membrane toughness.< Leer menos