Post-anthesis phosphorus (P) uptake and the remobilization of the previously acquired P are the principal sources of grain P nutrition in wheat. However, how the acquired P reaches the grains and its partitioning at the whole plant level remain poorly understood. Here, the temporal dynamics of the newly acquired P in durum wheat organs and its allocation to grain were examined using pulse-chase P-32-labeling experiments at 5 and 14 days after anthesis. Durum wheat plants were grown hydroponically under high and low P supplies. Each labeling experiment lasted for 24 h. Plants were harvested 24, 48, and 96 h after labeling. Low and high P treatments significantly affected the allocation of the newly acquired P at the whole plant level. Three days (96 h) after the first P-32-labeling, 8% and 4% of the newly acquired P from exogenous solution were allocated to grains, 73% and 55% to the remainder aboveground organs, and 19% and 41% to the roots at low and high P supplies, respectively. Three days after the second labeling, the corresponding values were 48% and 20% in grains, 44% and 53% in the remainder aboveground organs, and 8% and 27% in roots at low and high P supplies, respectively. These results reveal that the dynamics of P allocation to grain was faster in plants grown under low P supply than under high supply. However, the obtained results also indicate that the origin of P accumulated in durum wheat grains was mainly from P remobilization with little contribution from post-anthesis P uptake. The present study emphasizes the role of vegetative organs as temporary storage of P taken up during the grain filling period before its final allocation to grains.< Réduire