Aerated compost tea (ACT) contains soluble humic substances (SHS) that are expected to alter the dynamics and ecotoxicity of Cu in soil. This study investigated the efficiency of ACT in enhancing the mobility and phytoextraction of Cu in vineyard soil. Crimson clover (Trifolium incarnatum L.) was grown on a vineyard soil at three concentrations of Cu (90, 261 and 432 mg kg-1), and supplied (or not) with ACT, then sampled after 56 days to determine the amount of Cu phytoextracted. Soil was extracted with 0.01 M KCl and potentiometric analyses were performed to measure the impact of ACT on the speciation of Cu in the extraction solution. ACT was found to increase the mobility of Cu in the soil by a factor of 3-14 depending on the soil Cu content and on the soil extraction date. The increase in Cu mobility was associated with an increase in absorbance at 254 nm and with a decrease in the free ionic fraction of Cu in the KCl extract, suggesting that Cu was mainly mobilized by the SHS present in the compost tea, and through a ligand-controlled dissolution process. ACT increased Cu phytoextraction at Cu90 and Cu261 by on average 80% thanks to its positive impact on plant growth, and on Cu accumulation in plant shoots, whereas it reduced Cu phytoextraction at Cu432 due to its deleterious effect on plant growth at this soil Cu content. ACT is thus an efficient way to increase the phytoavailability of Cu in soil, but probably should not be used in vineyard soils that are highly contaminated by Cu. To obtain Cu phytoextraction yields in line with the needs of the wine sector, the use of ACT needs to be associated with the cultivation of a Cu-accumulating plant.< Réduire