Afficher la notice abrégée

dc.rights.licenseopenen_US
dc.contributor.authorGENDRE, Julia
hal.structure.identifierUnité de Recherche Œnologie [Villenave d'Ornon] [OENO]
dc.contributor.authorLE MARREC, Claire
dc.contributor.authorPETIT, Marie-Agnès
dc.contributor.authorCHAILLOU, Stéphane
dc.contributor.authorLANDAUD, Sophie
dc.contributor.authorDUGAT-BONY, Eric
dc.date.accessioned2024-03-12T14:33:58Z
dc.date.available2024-03-12T14:33:58Z
dc.date.conference2023-07-05
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/188721
dc.description.abstractEnContext/aim: Fermented food constitutes 30% of the human diet in average. The study of fermented foods of plant origin responds to industrial and economic issues. Indeed, fermentation failure is responsible for important economic losses and can lead to the production of food waste. Furthermore, the upcoming transition in our diet with the reduction of meat consumption and the increase of vegetable consumption leads to the great return of fermentation, a simple and energy energy-efficient food preservation process that generates tasty products with high nutritional value. The bacterial and fungal communities of fermented foods of plant origin (wine, cabbage, carrot, turnip) are well known, which is not the case for phage communities (i.e. bacterial viruses). The scientific challenges of our project are therefore to provide a detailed description of their composition and to better understand their role in this peculiar type of microbial ecosystems. The objective of the present study was to evaluate the impact of phages on the microbial dynamics of two different types of fermented foods of plant origin: sauerkraut (solid food) and wine (beverage). Method: The viral fraction of the food products collected at different time points during the fermentation process was purified by a specific method relying on the removal of microbial cells by centrifugation and filtration followed by viral particles’ concentration using polyethylene glycol (PEG). Viruses were quantified by epifluorescence microscopy and the composition of viral communities was determined through a viral metagenomics approach involving nucleic acids extraction, DNA and RNA amplification and high throughput sequencing. Results: Based on epifluorescence microscopy, we estimated the concentration of viruses at 5 x 107 particles/g of sauerkraut after fermentation and 107 particles/L of wine. Viral metagenomics analysis revealed that the most abundant viral contigs shared some sequence homology with phages known to infect the principal bacteria responsible for fermentation in the studied systems, for example Leuconostoc mesenteroides and Lactiplantibacillus plantarum in sauerkraut. Interestingly, few contigs slightly related to RNA phage genomes were detected for the first time in this type of ecosystem, shedding light onto potentially new viruses to be further characterized.
dc.language.isoENen_US
dc.publisherIseki-Food Associationen_US
dc.subject.enBacteriophages
dc.subject.enFermentated food
dc.subject.enMetagenomic
dc.title.enExploration of viral diversity in fermented foods of plant origin
dc.typeCommunication dans un congrèsen_US
dc.subject.halSciences du Vivant [q-bio]/Biologie végétaleen_US
bordeaux.hal.laboratoriesOenologie - UMR 1366en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionBordeaux INPen_US
bordeaux.institutionINRAEen_US
bordeaux.conference.title7th International ISEKI-Food Conferenceen_US
bordeaux.countryfren_US
bordeaux.conference.cityPalaiseauen_US
hal.invitedouien_US
hal.proceedingsouien_US
hal.conference.end2023-07-07
hal.popularnonen_US
hal.audienceInternationaleen_US
hal.exportfalse
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.au=GENDRE,%20Julia&LE%20MARREC,%20Claire&PETIT,%20Marie-Agn%C3%A8s&CHAILLOU,%20St%C3%A9phane&LANDAUD,%20Sophie&rft.genre=unknown


Fichier(s) constituant ce document

FichiersTailleFormatVue

Il n'y a pas de fichiers associés à ce document.

Ce document figure dans la(les) collection(s) suivante(s)

Afficher la notice abrégée