Afficher la notice abrégée

Integron cassettes integrate into bacterial genomes via widespread non-classical attG sites

dc.rights.licenseopenen_US
hal.structure.identifierPlasticité du Génome Bactérien - Bacterial Genome Plasticity [PGB]
dc.contributor.authorLOOT, Céline
hal.structure.identifierHub Bioinformatique et Biostatistique - Bioinformatics and Biostatistics HUB
dc.contributor.authorMILLOT, Gael
hal.structure.identifierPlasticité du Génome Bactérien - Bacterial Genome Plasticity [PGB]
hal.structure.identifierCollège Doctoral
dc.contributor.authorRICHARD, Egill
hal.structure.identifierGénomique évolutive des Microbes / Microbial Evolutionary Genomics
hal.structure.identifierCollège Doctoral
hal.structure.identifierCentre d'Études du Bouchet [DGA Maitrise NRBC]
dc.contributor.authorLITTNER, Eloi
hal.structure.identifierPlasticité du Génome Bactérien - Bacterial Genome Plasticity [PGB]
hal.structure.identifierCollège Doctoral
dc.contributor.authorVIT, Claire
hal.structure.identifierHub Bioinformatique et Biostatistique - Bioinformatics and Biostatistics HUB
dc.contributor.authorLEMOINE, Frédéric
hal.structure.identifierHub Bioinformatique et Biostatistique - Bioinformatics and Biostatistics HUB
dc.contributor.authorNÉRON, Bertrand
hal.structure.identifierLaboratoire de Recherche en Informatique [LRI]
hal.structure.identifierInria de Paris
dc.contributor.authorCURY, Jean
hal.structure.identifierPlasticité du Génome Bactérien - Bacterial Genome Plasticity [PGB]
hal.structure.identifierCollège Doctoral
dc.contributor.authorDARRACQ, Baptiste
hal.structure.identifierPlasticité du Génome Bactérien - Bacterial Genome Plasticity [PGB]
hal.structure.identifierCollège Doctoral
dc.contributor.authorNIAULT, Théophile
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
hal.structure.identifierViral DNA Integration and Chromatin Dynamics Network [DyNAVir]
dc.contributor.authorLAPAILLERIE, Delphine
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
hal.structure.identifierViral DNA Integration and Chromatin Dynamics Network [DyNAVir]
dc.contributor.authorPARISSI, Vincent
hal.structure.identifierGénomique évolutive des Microbes / Microbial Evolutionary Genomics
dc.contributor.authorROCHA, Eduardo
hal.structure.identifierPlasticité du Génome Bactérien - Bacterial Genome Plasticity [PGB]
dc.contributor.authorMAZEL, Didier
dc.date.accessioned2024-01-15T12:28:14Z
dc.date.available2024-01-15T12:28:14Z
dc.date.issued2024-01-03
dc.identifier.issn2058-5276en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/187165
dc.description.abstractEnIntegrons are genetic elements involved in bacterial adaptation which capture, shuffle and express genes encoding adaptive functions embedded in cassettes. These events are governed by the integron integrase through site-specific recombination between attC and attI integron sites. Using computational and molecular genetic approaches, here we demonstrate that the integrase also catalyses cassette integration into bacterial genomes outside of its known att sites. Once integrated, these cassettes can be expressed if located near bacterial promoters and can be excised at the integration point or outside, inducing chromosomal modifications in the latter case. Analysis of more than 5 × 10 5 independent integration events revealed a very large genomic integration landscape. We identified consensus recombination sequences, named attG sites, which differ greatly in sequence and structure from classical att sites. These results unveil an alternative route for dissemination of adaptive functions in bacteria and expand the role of integrons in bacterial evolution.
dc.description.sponsorshipOrganisation et montée en puissance d'une Infrastructure Nationale de Génomiqueen_US
dc.description.sponsorshipEtude de l'impact des intégrons chromosiques sur l'évolution des bactériesen_US
dc.description.sponsorshipIntegrative Biology of Emerging Infectious Diseasesen_US
dc.language.isoENen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc/
dc.subject.meshBacteria
dc.subject.meshGenome
dc.subject.meshGenomics
dc.subject.meshIntegrases
dc.subject.meshIntegrons
dc.title.enIntegron cassettes integrate into bacterial genomes via widespread non-classical attG sites
dc.typeArticle de revueen_US
dc.identifier.doi10.1038/s41564-023-01548-yen_US
dc.subject.halSciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biologie moléculaireen_US
dc.subject.halSciences du Vivant [q-bio]/Microbiologie et Parasitologieen_US
bordeaux.journalNature Microbiologyen_US
bordeaux.page228-240en_US
bordeaux.volume9en_US
bordeaux.hal.laboratoriesMFP (Laboratoire Microbiologie Fondamentale et Pathogénicité) - UMR 5234en_US
bordeaux.issue1en_US
bordeaux.institutionCNRSen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcehal
hal.identifierpasteur-04384854
hal.version1
hal.popularnonen_US
hal.audienceInternationaleen_US
hal.exportfalse
workflow.import.sourcehal
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Nature%20Microbiology&rft.date=2024-01-03&rft.volume=9&rft.issue=1&rft.spage=228-240&rft.epage=228-240&rft.eissn=2058-5276&rft.issn=2058-5276&rft.au=LOOT,%20C%C3%A9line&MILLOT,%20Gael&RICHARD,%20Egill&LITTNER,%20Eloi&VIT,%20Claire&rft.genre=article


Fichier(s) constituant ce document

Thumbnail
Nom:
MFP_Nat Microbiol_2024_Loot.pdf
Taille:
3.525Mo
Format:
PDF
Voir/Ouvrir

Ce document figure dans la(les) collection(s) suivante(s)

Afficher la notice abrégée