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Integron cassettes integrate into bacterial genomes via widespread non-classical attG sites
LITTNER, Eloi
Génomique évolutive des Microbes / Microbial Evolutionary Genomics
Collège Doctoral
Centre d'Études du Bouchet [DGA Maitrise NRBC]
Génomique évolutive des Microbes / Microbial Evolutionary Genomics
Collège Doctoral
Centre d'Études du Bouchet [DGA Maitrise NRBC]
DARRACQ, Baptiste
Plasticité du Génome Bactérien - Bacterial Genome Plasticity [PGB]
Collège Doctoral
Plasticité du Génome Bactérien - Bacterial Genome Plasticity [PGB]
Collège Doctoral
NIAULT, Théophile
Plasticité du Génome Bactérien - Bacterial Genome Plasticity [PGB]
Collège Doctoral
Plasticité du Génome Bactérien - Bacterial Genome Plasticity [PGB]
Collège Doctoral
LAPAILLERIE, Delphine
Microbiologie Fondamentale et Pathogénicité [MFP]
Viral DNA Integration and Chromatin Dynamics Network [DyNAVir]
Microbiologie Fondamentale et Pathogénicité [MFP]
Viral DNA Integration and Chromatin Dynamics Network [DyNAVir]
PARISSI, Vincent
Microbiologie Fondamentale et Pathogénicité [MFP]
Viral DNA Integration and Chromatin Dynamics Network [DyNAVir]
< Reduce
Microbiologie Fondamentale et Pathogénicité [MFP]
Viral DNA Integration and Chromatin Dynamics Network [DyNAVir]
Language
EN
Article de revue
This item was published in
Nature Microbiology. 2024-01-03, vol. 9, n° 1, p. 228-240
English Abstract
Integrons are genetic elements involved in bacterial adaptation which capture, shuffle and express genes encoding adaptive functions embedded in cassettes. These events are governed by the integron integrase through ...Read more >
Integrons are genetic elements involved in bacterial adaptation which capture, shuffle and express genes encoding adaptive functions embedded in cassettes. These events are governed by the integron integrase through site-specific recombination between attC and attI integron sites. Using computational and molecular genetic approaches, here we demonstrate that the integrase also catalyses cassette integration into bacterial genomes outside of its known att sites. Once integrated, these cassettes can be expressed if located near bacterial promoters and can be excised at the integration point or outside, inducing chromosomal modifications in the latter case. Analysis of more than 5 × 10 5 independent integration events revealed a very large genomic integration landscape. We identified consensus recombination sequences, named attG sites, which differ greatly in sequence and structure from classical att sites. These results unveil an alternative route for dissemination of adaptive functions in bacteria and expand the role of integrons in bacterial evolution.Read less <
ANR Project
Organisation et montée en puissance d'une Infrastructure Nationale de Génomique
Etude de l'impact des intégrons chromosiques sur l'évolution des bactéries - ANR-21-CE12-0002
Integrative Biology of Emerging Infectious Diseases - ANR-10-LABX-0062
Etude de l'impact des intégrons chromosiques sur l'évolution des bactéries - ANR-21-CE12-0002
Integrative Biology of Emerging Infectious Diseases - ANR-10-LABX-0062