LOOT, Céline; MILLOT, Gael; RICHARD, Egill; LITTNER, Eloi; VIT, Claire; LEMOINE, Frédéric; NÉRON, Bertrand; CURY, Jean; DARRACQ, Baptiste; NIAULT, Théophile; LAPAILLERIE, Delphine; PARISSI, Vincent; ROCHA, Eduardo; MAZEL, Didier

doi:10.1038/s41564-023-01548-y

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LOOT, Céline
Plasticité du Génome Bactérien - Bacterial Genome Plasticity [PGB]

MILLOT, Gael
Hub Bioinformatique et Biostatistique - Bioinformatics and Biostatistics HUB

RICHARD, Egill
Plasticité du Génome Bactérien - Bacterial Genome Plasticity [PGB]
Collège Doctoral

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Article de revue

Este ítem está publicado en

Nature Microbiology. 2024-01-03, vol. 9, n° 1, p. 228-240

Resumen en inglés

Integrons are genetic elements involved in bacterial adaptation which capture, shuffle and express genes encoding adaptive functions embedded in cassettes. These events are governed by the integron integrase through site-specific recombination between attC and attI integron sites. Using computational and molecular genetic approaches, here we demonstrate that the integrase also catalyses cassette integration into bacterial genomes outside of its known att sites. Once integrated, these cassettes can be expressed if located near bacterial promoters and can be excised at the integration point or outside, inducing chromosomal modifications in the latter case. Analysis of more than 5 × 10 5 independent integration events revealed a very large genomic integration landscape. We identified consensus recombination sequences, named attG sites, which differ greatly in sequence and structure from classical att sites. These results unveil an alternative route for dissemination of adaptive functions in bacteria and expand the role of integrons in bacterial evolution.< Leer menos

URI

https://oskar-bordeaux.fr/handle/20.500.12278/187165

DOI

http://dx.doi.org/10.1038/s41564-023-01548-y

Proyecto ANR

Organisation et montée en puissance d'une Infrastructure Nationale de Génomique - ANR-10-INBS-0009
Etude de l'impact des intégrons chromosiques sur l'évolution des bactéries
Integrative Biology of Emerging Infectious Diseases - ANR-10-LABX-0062

Centros de investigación

MFP (Laboratoire Microbiologie Fondamentale et Pathogénicité) - UMR 5234