Deciphering Immediate Post-Pulse Membrane Resealing from 4-Electrode Impedance Measurements by Numerical Modeling
COLLIN, Annabelle
Modélisation Mathématique pour l'Oncologie [MONC]
Institut de Mathématiques de Bordeaux [IMB]
Institut Polytechnique de Bordeaux [Bordeaux INP]
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Modélisation Mathématique pour l'Oncologie [MONC]
Institut de Mathématiques de Bordeaux [IMB]
Institut Polytechnique de Bordeaux [Bordeaux INP]
COLLIN, Annabelle
Modélisation Mathématique pour l'Oncologie [MONC]
Institut de Mathématiques de Bordeaux [IMB]
Institut Polytechnique de Bordeaux [Bordeaux INP]
Modélisation Mathématique pour l'Oncologie [MONC]
Institut de Mathématiques de Bordeaux [IMB]
Institut Polytechnique de Bordeaux [Bordeaux INP]
MIR, Lluis
Aspects métaboliques et systémiques de l'oncogénèse pour de nouvelles approches thérapeutiques [METSY]
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Aspects métaboliques et systémiques de l'oncogénèse pour de nouvelles approches thérapeutiques [METSY]
Langue
en
Article de revue
Ce document a été publié dans
Bioelectricity. 2023-12-01, vol. 5, n° 4, p. 266-278
Liebert
Résumé en anglais
Background and Objective: The present paper proposes a numerical strategy to decipher the dynamics of the cell membranes exposed to an electroporating electric field from bioimpedance measurements. In particular we aim at ...Lire la suite >
Background and Objective: The present paper proposes a numerical strategy to decipher the dynamics of the cell membranes exposed to an electroporating electric field from bioimpedance measurements. In particular we aim at discriminating between the increase of membrane conductivity due to electroporation from the increase of buffer conductivity due to ion exchange between buffer and cells. Methods: We propose first a robust calibration procedure that enables to account for the complexity of the 4--electrode experimental set-up. Thanks to this robust calibration, we deduce the impedance of the sample from the measurements. Then we propose a simple electrical circuit model of the set-up, which is calibrated into two steps.Results: First we estimate the model parameters before the electroporating electric field, in order to obtain the cell parameters. The dynamic of the membrane resistance after the pulses is then calibrated simultaneously with the increase of the buffer conductivity due to ions exchanges. Interestingly, our model and our calibration strategy enable us to capture the dynamics of the cell membranes within a few seconds after the pulse. For longer times, we explain how additional measurements of the buffer conductivity should be performed to track the dynamics of the membrane resealing more accurately.Conclusions: The combination of the robust calibration with the well-designed equivalent circuit model enables us to capture the dynamics of ions exchange and membrane permeabilisation within the few seconds after the electric pulse.< Réduire
Mots clés en anglais
Electrical modeling of cells
Numerical Optimization
Electroporation
Impedan- cemetry
4-electrode measurements
Impedancemetry
Origine
Importé de halUnités de recherche