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Deciphering Immediate Post-Pulse Membrane Resealing from 4-Electrode Impedance Measurements by Numerical Modeling

hal.structure.identifierModélisation Mathématique pour l'Oncologie [MONC]
hal.structure.identifierInstitut de Mathématiques de Bordeaux [IMB]
hal.structure.identifierInstitut Polytechnique de Bordeaux [Bordeaux INP]
dc.contributor.authorCOLLIN, Annabelle
hal.structure.identifierUniversitat Pompeu Fabra [Barcelona] [UPF]
dc.contributor.authorGARCÍA-SÁNCHEZ, Tomás
hal.structure.identifierÉquipe Calcul scientifique et Modélisation
dc.contributor.authorCORRIDORE, Sergio
hal.structure.identifierAspects métaboliques et systémiques de l'oncogénèse pour de nouvelles approches thérapeutiques [METSY]
dc.contributor.authorMIR, Lluis
hal.structure.identifierModélisation Mathématique pour l'Oncologie [MONC]
dc.contributor.authorPOIGNARD, Clair
dc.date.issued2023-12-01
dc.identifier.issn2576-3105
dc.description.abstractEnBackground and Objective: The present paper proposes a numerical strategy to decipher the dynamics of the cell membranes exposed to an electroporating electric field from bioimpedance measurements. In particular we aim at discriminating between the increase of membrane conductivity due to electroporation from the increase of buffer conductivity due to ion exchange between buffer and cells. Methods: We propose first a robust calibration procedure that enables to account for the complexity of the 4--electrode experimental set-up. Thanks to this robust calibration, we deduce the impedance of the sample from the measurements. Then we propose a simple electrical circuit model of the set-up, which is calibrated into two steps.Results: First we estimate the model parameters before the electroporating electric field, in order to obtain the cell parameters. The dynamic of the membrane resistance after the pulses is then calibrated simultaneously with the increase of the buffer conductivity due to ions exchanges. Interestingly, our model and our calibration strategy enable us to capture the dynamics of the cell membranes within a few seconds after the pulse. For longer times, we explain how additional measurements of the buffer conductivity should be performed to track the dynamics of the membrane resealing more accurately.Conclusions: The combination of the robust calibration with the well-designed equivalent circuit model enables us to capture the dynamics of ions exchange and membrane permeabilisation within the few seconds after the electric pulse.
dc.language.isoen
dc.publisherLiebert
dc.rights.urihttp://creativecommons.org/licenses/by/
dc.subject.enElectrical modeling of cells
dc.subject.enNumerical Optimization
dc.subject.enElectroporation
dc.subject.enImpedan- cemetry
dc.subject.en4-electrode measurements
dc.subject.enImpedancemetry
dc.title.enDeciphering Immediate Post-Pulse Membrane Resealing from 4-Electrode Impedance Measurements by Numerical Modeling
dc.typeArticle de revue
dc.identifier.doi10.1089/bioe.2023.0012
dc.subject.halMathématiques [math]
bordeaux.journalBioelectricity
bordeaux.page266-278
bordeaux.volume5
bordeaux.issue4
bordeaux.peerReviewedoui
hal.identifierhal-04361087
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-04361087v1
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