Single α-particle irradiation permits real-time visualization of RNF8 accumulation at DNA damaged sites
Langue
en
Article de revue
Ce document a été publié dans
Scientific Reports. 2017, vol. 7, n° 1, p. 41764
Nature Publishing Group
Résumé en anglais
As well as being a significant source of environmental radiation exposure, α-particles are increasingly considered for use in targeted radiation therapy. A better understanding of α-particle induced damage at the DNA scale ...Lire la suite >
As well as being a significant source of environmental radiation exposure, α-particles are increasingly considered for use in targeted radiation therapy. A better understanding of α-particle induced damage at the DNA scale can be achieved by following their tracks in real-time in targeted living cells. Focused α-particle microbeams can facilitate this but, due to their low energy (up to a few MeV) and limited range, α-particles detection, delivery, and follow-up observations of radiation-induced damage remain difficult. In this study, we developed a thin Boron-doped Nano-Crystalline Diamond membrane that allows reliable single α-particles detection and single cell irradiation with negligible beam scattering. The radiation-induced responses of single 3 MeV α-particles delivered with focused microbeam are visualized in situ over thirty minutes after irradiation by the accumulation of the GFP-tagged RNF8 protein at DNA damaged sites.< Réduire
Mots clés en anglais
instrumentation
nuclear instrumentation
ionizing radiation
radioactivity
alpha-rays
radiotherapy
alpha particles
radiation-induced damage
diamond
boron doping
nano-crystalline diamond
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