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Rapid Adenovirus typing method for species identification.
| dc.rights.license | open | en_US |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | RAYNE, Fabienne | |
| hal.structure.identifier | Bordeaux population health [BPH] | |
| dc.contributor.author | WITTKOP, Linda | |
| dc.contributor.author | BADER, Clement | |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | KASSAB, Somar | |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | TUMIOTTO, Camille | |
| dc.contributor.author | BERCIAUD, Sylvie | |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | WODRICH, Harald | |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | LAFON, Marie-Edith | |
| dc.date.accessioned | 2023-11-13T13:54:52Z | |
| dc.date.available | 2023-11-13T13:54:52Z | |
| dc.date.issued | 2017-11-01 | |
| dc.identifier.issn | 1879-0984 | en_US |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/184739 | |
| dc.description.abstractEn | Adenoviruses are characterized by a large variability, reflected by their classification in species A to G. Certain species, eg A and C, could be associated with increased clinical severity, both in immunocompetent and immunocompromised hosts suggesting that in some instances species identification provides clinically relevant information. Here we designed a novel "pVI rapid typing method" to obtain quick, simple and cost effective species assignment for Adenoviruses, thanks to combined fusion temperature (Tm) and amplicon size analysis. Rapid typing results were compared to Sanger sequencing in the hexon gene for 140 Adenovirus-positive clinical samples included in the Typadeno study. Species A and C could be identified with a 100% positive predictive value, thus confirming the value of this simple typing method. | |
| dc.language.iso | EN | en_US |
| dc.subject.en | Adenovirus Infections | |
| dc.subject.en | Human | |
| dc.subject.en | Adenoviruses | |
| dc.subject.en | Human | |
| dc.subject.en | DNA Primers | |
| dc.subject.en | Genotyping Techniques | |
| dc.subject.en | Humans | |
| dc.subject.en | Immunocompetence | |
| dc.subject.en | Immunocompromised Host | |
| dc.subject.en | Polymerase Chain Reaction | |
| dc.subject.en | Predictive Value of Tests | |
| dc.subject.en | Sensitivity and Specificity | |
| dc.subject.en | Sequence Analysis | |
| dc.subject.en | DNA | |
| dc.subject.en | Transition Temperature | |
| dc.title.en | Rapid Adenovirus typing method for species identification. | |
| dc.title.alternative | J Virol Methods | en_US |
| dc.type | Article de revue | en_US |
| dc.identifier.doi | 10.1016/j.jviromet.2017.09.009 | en_US |
| dc.subject.hal | Sciences du Vivant [q-bio]/Microbiologie et Parasitologie | en_US |
| dc.identifier.pubmed | 28918074 | en_US |
| bordeaux.journal | Journal of Virological Methods | en_US |
| bordeaux.page | 156-160 | en_US |
| bordeaux.volume | 249 | en_US |
| bordeaux.hal.laboratories | MFP (Laboratoire Microbiologie Fondamentale et Pathogénicité) - UMR 5234 | en_US |
| bordeaux.institution | CNRS | en_US |
| bordeaux.peerReviewed | oui | en_US |
| bordeaux.inpress | non | en_US |
| bordeaux.import.source | pubmed | |
| hal.identifier | hal-04282538 | |
| hal.version | 1 | |
| hal.date.transferred | 2023-11-13T13:54:54Z | |
| hal.popular | non | en_US |
| hal.audience | Internationale | en_US |
| hal.export | true | |
| workflow.import.source | pubmed | |
| dc.rights.cc | Pas de Licence CC | en_US |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Journal%20of%20Virological%20Methods&rft.date=2017-11-01&rft.volume=249&rft.spage=156-160&rft.epage=156-160&rft.eissn=1879-0984&rft.issn=1879-0984&rft.au=RAYNE,%20Fabienne&WITTKOP,%20Linda&BADER,%20Clement&KASSAB,%20Somar&TUMIOTTO,%20Camille&rft.genre=article |
