TREBBIA, J.-B; BABY, R; TAMARAT, P.; LOUNIS, B.

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TREBBIA, J.-B
Laboratoire Photonique, Numérique et Nanosciences [LP2N]

BABY, R
Laboratoire Photonique, Numérique et Nanosciences [LP2N]

TAMARAT, P.
Laboratoire Photonique, Numérique et Nanosciences [LP2N]

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Optics Express. 2019

Optical Society of America - OSA Publishing

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2019

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We present a 3D fluorescence nanoscopy method operating at cryogenic temperatures, based on optical saturation of the excited state of individual molecules. Using a focused laser beam structured with a zero-intensity central region surrounded by intensity gradients in the three space directions, we achieve a sub-30 nm 3D optical resolution. Moreover, the analysis of the fluorescence scanning images of single molecules reveals the 3D orientation of their transition dipole with an accuracy of a few degrees. This method provides a valuable tool for locating neighboring molecules with overlapping optical transitions in order to study their interactions.< Leer menos

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3D optical nanoscopy with excited state saturation at liquid helium temperatures

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