BON, Pierre; BOURG, Nicolas; LÉCART, Sandrine; MONNERET, Serge; FORT, Emmanuel; WENGER, Jérôme; LÉVÊQUE-FORT, Sandrine

doi:10.1038/ncomms8764

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BON, Pierre
Laboratoire Photonique, Numérique et Nanosciences [LP2N]
Institut Langevin - Ondes et Images (UMR7587) [IL]
Institut des Sciences Moléculaires d'Orsay [ISMO]

BOURG, Nicolas
Institut des Sciences Moléculaires d'Orsay [ISMO]

LÉCART, Sandrine
Centre de Photonique Biomédicale [CPBM]

Langue

Article de revue

Ce document a été publié dans

Nature Communications. 2015-07-27, vol. 6, p. 7764

Nature Publishing Group

Résumé en anglais

Meeting the nanometre resolution promised by super-resolution microscopy techniques (pointillist: PALM, STORM, scanning: STED) requires stabilizing the sample drifts in real time during the whole acquisition process. Metal nanoparticles are excellent probes to track the lateral drifts as they provide crisp and photostable information. However, achieving nano-metre axial super-localization is still a major challenge, as diffraction imposes large depths-of-fields. Here we demonstrate fast full three-dimensional nanometre super-localization of gold nanoparticles through simultaneous intensity and phase imaging with a wavefront-sensing camera based on quadriwave lateral shearing interferometry. We show how to combine the intensity and phase information to provide the key to the third axial dimension. Presently, we demonstrate even in the occurrence of large three-dimensional fluctuations of several microns, unprecedented sub-nanometre localization accuracies down to 0.7 nm in lateral and 2.7 nm in axial directions at 50 frames per second. We demonstrate that nanoscale stabilization greatly enhances the image quality and resolution in direct stochastic optical reconstruction microscopy imaging.< Réduire

Mots clés en anglais

Nanoparticles

Super-resolution microscopy

URI

https://oskar-bordeaux.fr/handle/20.500.12278/181663

DOI

http://dx.doi.org/10.1038/ncomms8764

Project ANR

Institut Langevin : Ondes et Images, du Fondamental à l'Innovation - ANR-10-LABX-0024
Paris Sciences et Lettres - ANR-10-IDEX-0001
Microscopie de fluorescence pour l'imagerie membranaire - ANR-11-EMMA-0047

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Importé de hal

Unités de recherche

Laboratoire Photonique, Numérique et Nanosciences (LP2N) - UMR 5298