PAVIOLO, Chiara; FERREIRA, Joana; LEE, Antony; HUNTER, Daniel; CALARESU, Ivo; NANDI, Somen; GROC, Laurent; COGNET, Laurent

doi:10.1021/acs.nanolett.1c04259

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PAVIOLO, Chiara
Laboratoire Photonique, Numérique et Nanosciences [LP2N]

FERREIRA, Joana
Interdisciplinary Institute for Neuroscience [Bordeaux] [IINS]

LEE, Antony
Laboratoire Photonique, Numérique et Nanosciences [LP2N]

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Nano Letters. 2022-08-29, vol. 22, n° 17, p. 6849 - 6856

American Chemical Society

Résumé en anglais

We provide evidence of a local synaptic nanoenvironment in the brain extracellular space (ECS) lying within 500 nm of postsynaptic densities. To reveal this brain compartment, we developed a correlative imaging approach dedicated to thick brain tissue based on single-particle tracking of individual fluorescent single wall carbon nanotubes (SWCNTs) in living samples and on speckle-based HiLo microscopy of synaptic labels. We show that the extracellular space around synapses bears specific properties in terms of morphology at the nanoscale and inner diffusivity. We finally show that the ECS juxta-synaptic region changes its diffusion parameters in response to neuronal activity, indicating that this nanoenvironment might play a role in the regulation of brain activity.< Réduire

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Single-walled carbon nanotubes

brain extracellular space

single particle tracking

superlocalization microscopy

organotypic brain slices

synapses

correlative imaging

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Near-Infrared Carbon Nanotube Tracking Reveals the Nanoscale Extracellular Space around Synapses

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