KORTEN, Till; NITZSCHE, Bert; GELL, Chris; RUHNOW, Felix; LEDUC, Cecile; DIEZ, Stefan

doi:10.1007/978-1-61779-282-3_7

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KORTEN, Till
Max Planck Institute of Molecular Cell Biology and Genetics [MPI-CBG]

NITZSCHE, Bert
Max Planck Institute of Molecular Cell Biology and Genetics [MPI-CBG]

GELL, Chris
Max Planck Institute of Molecular Cell Biology and Genetics [MPI-CBG]

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Methods in Molecular Biology. 2011-12-07, vol. 738, p. 121

Humana Press/Springer Imprint

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Recent developments in optical microscopy and nanometer tracking have greatly improved our understanding of cytoskeletal motor proteins. Using fluorescence microscopy, dynamic interactions are now routinely observed in vitro on the level of single molecules mainly using a geometry, where fluorescently labeled motors move on surface-immobilized filaments. In this chapter, we review recent methods related to single-molecule kinesin motility assays. In particular, we aim to provide practical advice on: how to set up the assays, how to acquire high-precision data from fluorescently labeled kinesin motors and attached quantum dots, and how to analyze data by nanometer tracking.< Réduire

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Fluorescence imaging of single Kinesin motors on immobilized microtubules.

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