A soft cortex is essential for asymmetric spindle positioning in mouse oocytes
| hal.structure.identifier | Centre interdisciplinaire de recherche en biologie [CIRB] | |
| dc.contributor.author | CHAIGNE, Agathe | |
| hal.structure.identifier | Institut Lumière Matière [Villeurbanne] [ILM] | |
| hal.structure.identifier | Laboratoire Photonique, Numérique et Nanosciences [LP2N] | |
| dc.contributor.author | CAMPILLO, Clément | |
| hal.structure.identifier | Weizmann Institute of Science [Rehovot, Israël] | |
| dc.contributor.author | GOV, Nir | |
| hal.structure.identifier | Laboratoire de Physique Théorique de la Matière Condensée [LPTMC] | |
| dc.contributor.author | VOITURIEZ, Raphael | |
| hal.structure.identifier | Centre interdisciplinaire de recherche en biologie [CIRB] | |
| dc.contributor.author | AZOURY, Jessica | |
| hal.structure.identifier | Centre interdisciplinaire de recherche en biologie [CIRB] | |
| dc.contributor.author | UMANA-DIAZ, Claudia | |
| hal.structure.identifier | Centre interdisciplinaire de recherche en biologie [CIRB] | |
| dc.contributor.author | ALMONACID, Maria | |
| hal.structure.identifier | Centre interdisciplinaire de recherche en biologie [CIRB] | |
| dc.contributor.author | QUEGUINER, Isabelle | |
| hal.structure.identifier | Physico-Chimie-Curie [PCC] | |
| hal.structure.identifier | Laboratoire Photonique, Numérique et Nanosciences [LP2N] | |
| dc.contributor.author | NASSOY, Pierre | |
| hal.structure.identifier | Laboratoire Physico-Chimie Curie [Institut Curie] [PCC] | |
| dc.contributor.author | SYKES, C. | |
| hal.structure.identifier | Centre interdisciplinaire de recherche en biologie [CIRB] | |
| dc.contributor.author | VERLHAC, Marie-Hélène | |
| hal.structure.identifier | Centre interdisciplinaire de recherche en biologie [CIRB] | |
| dc.contributor.author | TERRET, Marie-Emilie | |
| dc.date.accessioned | 2023-05-12T10:19:43Z | |
| dc.date.available | 2023-05-12T10:19:43Z | |
| dc.date.created | 2013-01-02 | |
| dc.date.issued | 2013-08-01 | |
| dc.identifier.issn | 1465-7392 | |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/181056 | |
| dc.description.abstractEn | At mitosis onset, cortical tension increases and cells round up, ensuring correct spindle morphogenesis and orientation. Thus, cortical tension sets up the geometric requirements of cell division. On the contrary, cortical tension decreases during meiotic divisions in mouse oocytes, a puzzling observation because oocytes are round cells, stable in shape, that actively position their spindles. We investigated the pathway leading to reduction in cortical tension and its significance for spindle positioning. We document a previously uncharacterized Arp2/3-dependent thickening of the cortical F-actin essential for first meiotic spindle migration to the cortex. Using micropipette aspiration, we show that cortical tension decreases during meiosis I, resulting from myosin-II exclusion from the cortex, and that cortical F-actin thickening promotes cortical plasticity. These events soften and relax the cortex. They are triggered by the Mos-MAPK pathway and coordinated temporally. Artificial cortex stiffening and theoretical modelling demonstrate that a soft cortex is essential for meiotic spindle positioning. | |
| dc.language.iso | en | |
| dc.publisher | Nature Publishing Group | |
| dc.subject.en | ARP2/3 COMPLEX | |
| dc.subject.en | CORTICAL TENSION | |
| dc.subject.en | SYMMETRY-BREAKING | |
| dc.subject.en | MICROTUBULE ORGANIZATION | |
| dc.subject.en | MEIOTIC SPINDLE | |
| dc.subject.en | MAP KINASE | |
| dc.subject.en | F-ACTIN MESHWORK | |
| dc.subject.en | :LIGHT-CHAIN KINASE | |
| dc.subject.en | MAMMALIAN OOCYTES | |
| dc.subject.en | SURFACE-RECEPTORS | |
| dc.title.en | A soft cortex is essential for asymmetric spindle positioning in mouse oocytes | |
| dc.type | Article de revue | |
| dc.identifier.doi | 10.1038/ncb2799 | |
| dc.subject.hal | Physique [physics]/Physique [physics]/Biophysique [physics.bio-ph] | |
| bordeaux.journal | Nature Cell Biology | |
| bordeaux.page | 958-966 | |
| bordeaux.volume | 15 | |
| bordeaux.hal.laboratories | Laboratoire Photonique, Numérique et Nanosciences (LP2N) - UMR 5298 | * |
| bordeaux.issue | 8 | |
| bordeaux.institution | Université de Bordeaux | |
| bordeaux.institution | CNRS | |
| bordeaux.peerReviewed | oui | |
| hal.identifier | hal-00994646 | |
| hal.version | 1 | |
| hal.origin.link | https://hal.archives-ouvertes.fr//hal-00994646v1 | |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Nature%20Cell%20Biology&rft.date=2013-08-01&rft.volume=15&rft.issue=8&rft.spage=958-966&rft.epage=958-966&rft.eissn=1465-7392&rft.issn=1465-7392&rft.au=CHAIGNE,%20Agathe&CAMPILLO,%20Cl%C3%A9ment&GOV,%20Nir&VOITURIEZ,%20Raphael&AZOURY,%20Jessica&rft.genre=article |
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