LAMBERT, Francois; CARDOIT, Laura; COURTY, Elric; BOUGEROL, Marion; THOBY-BRISSON, Muriel; SIMMERS, John; TOSTIVINT, Herve; LE RAY, Didier

doi:10.7554/elife.30693

Institut de Neurosciences cognitives et intégratives d'Aquitaine [INCIA]

CARDOIT, Laura
Institut de Neurosciences cognitives et intégratives d'Aquitaine [INCIA]

COURTY, Elric
Institut de Neurosciences cognitives et intégratives d'Aquitaine [INCIA]

Langue

Article de revue

Ce document a été publié dans

eLife. 2018-05-30

Résumé en anglais

In vertebrates, functional motoneurons are defined as differentiated neurons that are connected to a central premotor network and activate peripheral muscle using acetylcholine. Generally, motoneurons and muscles develop simultaneously during embryogenesis. However, during Xenopus metamorphosis, developing limb motoneurons must reach their target muscles through the already established larval cholinergic axial neuromuscular system. Here, we demonstrate that at metamorphosis onset, spinal neurons retrogradely labeled from the emerging hindlimbs initially express neither choline acetyltransferase nor vesicular acetylcholine transporter. Nevertheless, they are positive for the motoneuronal transcription factor Islet1/2 and exhibit intrinsic and axial locomotor-driven electrophysiological activity. Moreover, the early appendicular motoneurons activate developing limb muscles via nicotinic antagonist-resistant, glutamate antagonist-sensitive, neuromuscular synapses. Coincidently, the hindlimb muscles transiently express glutamate, but not nicotinic receptors. Subsequently, both pre- and postsynaptic neuromuscular partners switch definitively to typical cholinergic transmitter signaling. Thus, our results demonstrate a novel context-dependent re-specification of neurotransmitter phenotype during neuromuscular system development.< Réduire

URI

https://oskar-bordeaux.fr/handle/20.500.12278/173234

DOI

http://dx.doi.org/10.7554/elife.30693

Unités de recherche

Institut de neurosciences cognitives et intégratives d'Aquitaine (INCIA) - UMR 5287

Voir/Ouvrir

Métadonnées

Partager cette publication !

Licence d’utilisation du document

Functional limb muscle innervation prior to cholinergic transmitter specification during early metamorphosis in Xenopus

Langue

Ce document a été publié dans

Résumé en anglais

URI

DOI

Unités de recherche