Show simple item record

dc.rights.licenseopenen_US
dc.contributor.authorPIQUET, Leo
dc.contributor.authorCOUTANT, Kelly
dc.contributor.authorMITCHELL, Andrew
dc.contributor.authorBEN ANES, Amel
dc.contributor.authorBOLLMANN, Enola
dc.contributor.authorSCHOONJANS, Nathan
dc.contributor.authorBERUBE, Julie
dc.contributor.authorBORDELEAU, Francois
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorBRISSON, Alain
dc.contributor.authorLANDREVILLE, Solange
dc.date.accessioned2023-03-28T08:34:17Z
dc.date.available2023-03-28T08:34:17Z
dc.date.issued2022-01
dc.identifier.issn2073-4409en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/172637
dc.description.abstractEnUveal melanoma (UM) is the most common primary intraocular tumor and often spreads to the liver. Intercellular communication though extracellular vesicles (EVs) plays an important role in several oncogenic processes, including metastasis, therapeutic resistance, and immune escape. This study examines how EVs released by UM cells modify stellate and endothelial cells in the tumor microenvironment. The surface markers, and the concentration and size of EVs derived from UM cells or choroidal melanocytes were characterized by high-resolution flow cytometry, electron microscopy, and Western blotting. The selective biodistribution of EVs was studied in mice by fluorescence imaging. The activation/contractility of stellate cells and the tubular organization of endothelial cells after exposure to melanomic EVs were determined by traction force microscopy, collagen gel contraction, or endothelial tube formation assays. We showed that large EVs from UM cells and healthy melanocytes are heterogenous in size, as well as their expression of phosphatidylserine, tetraspanins, and Tsg101. Melanomic EVs mainly accumulated in the liver and lungs of mice. Hepatic stellate cells with internalized melanomic EVs had increased contractility, whereas EV-treated endothelial cells developed more capillary-like networks. Our study demonstrates that the transfer of EVs from UM cells leads to a pro-fibrotic and pro-angiogenic phenotype in hepatic stellate and endothelial cells.
dc.language.isoENen_US
dc.rightsAttribution 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/us/*
dc.subject.enExtracellular vesicles
dc.subject.enMetastatic uveal melanoma
dc.subject.enHepatic stellate cells
dc.subject.enEndothelial cells
dc.title.enExtracellular Vesicles from Ocular Melanoma Have Pro-Fibrotic and Pro-Angiogenic Properties on the Tumor Microenvironment
dc.typeArticle de revueen_US
dc.identifier.doi10.3390/cells11233828en_US
dc.subject.halChimie/Matériauxen_US
bordeaux.journalCellsen_US
bordeaux.volume11en_US
bordeaux.hal.laboratoriesCBMN : Chimie & de Biologie des Membranes & des Nano-objets - UMR 5248en_US
bordeaux.issue23en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionBordeaux INPen_US
bordeaux.institutionCNRSen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
hal.identifierhal-04048650
hal.version1
hal.date.transferred2023-03-28T08:34:34Z
hal.exporttrue
dc.rights.ccCC BYen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Cells&rft.date=2022-01&rft.volume=11&rft.issue=23&rft.eissn=2073-4409&rft.issn=2073-4409&rft.au=PIQUET,%20Leo&COUTANT,%20Kelly&MITCHELL,%20Andrew&BEN%20ANES,%20Amel&BOLLMANN,%20Enola&rft.genre=article


Files in this item

Thumbnail
Thumbnail

This item appears in the following Collection(s)

Show simple item record