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dc.rights.licenseopenen_US
dc.contributor.authorMILES, James
dc.contributor.authorSOUBEYRAN, Isabelle
dc.contributor.authorMARLIOT, Florence
dc.contributor.authorPANGON, Nicolas
dc.contributor.authorITALIANO, Antoine
hal.structure.identifierBordeaux population health [BPH]
dc.contributor.authorBELLERA, Carine
dc.contributor.authorWARD, Stephen G.
dc.contributor.authorPAGES, Franck
dc.contributor.authorPALUSSIERE, Jean
dc.contributor.authorLARIJANI, Banafshe
dc.date.accessioned2023-02-17T10:21:22Z
dc.date.available2023-02-17T10:21:22Z
dc.date.issued2022-11-22
dc.identifier.issn2072-6694 (Print) 2072-6694 (Electronic) 2072-6694 (Linking)en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/171982
dc.description.abstractEnBACKGROUND: Cases of the spontaneous regression of multiple pulmonary metastases, after radiofrequency ablation (RFA), of a single lung metastasis, have been documented to be mediated by the immune system. The interaction of immune checkpoints, e.g., PD-1/PD-L1 and CTLA-4/CD80, may explain this phenomenon. The purpose of this study is to identify and quantify immune mechanisms triggered by RFA of pulmonary metastases originating from colorectal cancer. METHODS: We used two-site time-resolved Förster resonance energy transfer as determined by frequency-domain FLIM (iFRET) for the quantification of receptor-ligand interactions. iFRET provides a method by which immune checkpoint interaction states can be quantified in a spatiotemporal manner. The same patient sections were used for assessment of ligand-receptor interaction and intratumoral T-cell labeling. CONCLUSION: The checkpoint interaction states quantified by iFRET did not correlate with ligand expression. We show that immune checkpoint ligand expression as a predictive biomarker may be unsuitable as it does not confirm checkpoint interactions. In pre-RFA-treated metastases, there was a significant and negative correlation between PD-1/PD-L1 interaction state and intratumoral CD3+ and CD8+ density. The negative correlation of CD8+ and interactive states of PD-1/PD-L1 can be used to assess the state of immune suppression in RFA-treated patients.
dc.language.isoENen_US
dc.rightsAttribution 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/us/*
dc.subject.enImmune checkpoints
dc.subject.enPD-1/PD-L1
dc.subject.enCTLA-4/CD80
dc.subject.enRadiofrequency ablation
dc.subject.enAbscopal effect
dc.subject.enFRET/FLIM
dc.subject.enImmune surveyance
dc.title.enDetermination of Interactive States of Immune Checkpoint Regulators in Lung Metastases after Radiofrequency Ablation
dc.title.alternativeCancers (Basel)en_US
dc.typeArticle de revueen_US
dc.identifier.doi10.3390/cancers14235738en_US
dc.subject.halSciences du Vivant [q-bio]/Santé publique et épidémiologieen_US
dc.identifier.pubmed36497220en_US
bordeaux.journalCancersen_US
bordeaux.volume14en_US
bordeaux.hal.laboratoriesBordeaux Population Health Research Center (BPH) - UMR 1219en_US
bordeaux.issue23en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionINSERMen_US
bordeaux.teamEPICENE_BPHen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
hal.identifierhal-03993898
hal.version1
hal.date.transferred2023-02-17T10:21:26Z
hal.exporttrue
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Cancers&rft.date=2022-11-22&rft.volume=14&rft.issue=23&rft.eissn=2072-6694%20(Print)%202072-6694%20(Electronic)%202072-6694%20(Linking)&rft.issn=2072-6694%20(Print)%202072-6694%20(Electronic)%202072-6694%20(Linking)&rft.au=MILES,%20James&SOUBEYRAN,%20Isabelle&MARLIOT,%20Florence&PANGON,%20Nicolas&ITALIANO,%20Antoine&rft.genre=article


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