MILES, James; SOUBEYRAN, Isabelle; MARLIOT, Florence; PANGON, Nicolas; ITALIANO, Antoine; BELLERA, Carine; WARD, Stephen G.; PAGES, Franck; PALUSSIERE, Jean; LARIJANI, Banafshe

doi:10.3390/cancers14235738

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Ce document a été publié dans

Cancers. 2022-11-22, vol. 14, n° 23

Résumé en anglais

BACKGROUND: Cases of the spontaneous regression of multiple pulmonary metastases, after radiofrequency ablation (RFA), of a single lung metastasis, have been documented to be mediated by the immune system. The interaction of immune checkpoints, e.g., PD-1/PD-L1 and CTLA-4/CD80, may explain this phenomenon. The purpose of this study is to identify and quantify immune mechanisms triggered by RFA of pulmonary metastases originating from colorectal cancer. METHODS: We used two-site time-resolved Förster resonance energy transfer as determined by frequency-domain FLIM (iFRET) for the quantification of receptor-ligand interactions. iFRET provides a method by which immune checkpoint interaction states can be quantified in a spatiotemporal manner. The same patient sections were used for assessment of ligand-receptor interaction and intratumoral T-cell labeling. CONCLUSION: The checkpoint interaction states quantified by iFRET did not correlate with ligand expression. We show that immune checkpoint ligand expression as a predictive biomarker may be unsuitable as it does not confirm checkpoint interactions. In pre-RFA-treated metastases, there was a significant and negative correlation between PD-1/PD-L1 interaction state and intratumoral CD3+ and CD8+ density. The negative correlation of CD8+ and interactive states of PD-1/PD-L1 can be used to assess the state of immune suppression in RFA-treated patients.< Réduire

Mots clés en anglais

Immune checkpoints

PD-1/PD-L1

CTLA-4/CD80

Radiofrequency ablation

Abscopal effect

FRET/FLIM

Immune surveyance

URI

https://oskar-bordeaux.fr/handle/20.500.12278/171982

DOI

http://dx.doi.org/10.3390/cancers14235738

Unités de recherche

Bordeaux Population Health Research Center (BPH) - UMR 1219

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Determination of Interactive States of Immune Checkpoint Regulators in Lung Metastases after Radiofrequency Ablation

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