MALIVERT, Maxime; HARMS, Fabrice; VEILLY, Cynthia; LEGRAND, Jerome; LI, Ziqiang; BAYER, Emmanuelle; CHOQUET, Daniel; DUCROS, Mathieu

doi:10.1364/BOE.471757

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MALIVERT, Maxime
Bordeaux Imaging Center [BIC]

HARMS, Fabrice

VEILLY, Cynthia

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Ce document a été publié dans

Biomedical optics express. 2022-12-01, vol. 13, n° 12, p. 6211-6228

Résumé en anglais

Lattice light-sheet microscopy (LLSM) is a very efficient technique for high resolution 3D imaging of dynamic phenomena in living biological samples. However, LLSM imaging remains limited in depth due to optical aberrations caused by sample-based refractive index mismatch. Here, we propose a simple and low-cost active image optimization (AIO) method to recover high resolution imaging inside thick biological samples. AIO is based on (1) a light-sheet autofocus step (AF) followed by (2) an adaptive optics image-based optimization. We determine the optimum AIO parameters to provide a fast, precise and robust aberration correction on biological samples. Finally, we demonstrate the performances of our approach on sub-micrometric structures in brain slices and plant roots.< Réduire

URI

https://oskar-bordeaux.fr/handle/20.500.12278/171799

DOI

http://dx.doi.org/10.1364/BOE.471757

Projet Européen

The function of membrane tethering in plant intercellular communication
AMPA Receptor Dynamic Organization and Synaptic transmission in health and disease

Unités de recherche

Laboratoire de Biogenèse Membranaire (LBM) - UMR 5200