BERGER, Margot M. J.; LAILHEUGUE, Vincent; ZHELYAZOVA, Gergana; STAMMITTI, Linda; LAUVERGEAT, Virginie; BARRIEU, Francois; GALLUSCI, Philippe

doi:10.20870/oeno-one.2022.56.2.5000

BERGER, Margot M. J.
Ecophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV]

LAILHEUGUE, Vincent
Ecophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV]

ZHELYAZOVA, Gergana
Ecophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV]

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Article de revue

Ce document a été publié dans

Oeno One. 2022-04-06, vol. 56, n° 2, p. 1-13

Résumé en anglais

Recent advances in our understanding of plant physiology and adaptation to the environment are tightly related to the development of ‘omics’ technologies such as metabolomics, transcriptomics, genomics and epigenomics that allow a more comprehensive view of the plant functioning. In this context, the ability to extract DNA and RNA from small amounts of plant material can be a limiting factor, worse in the case of non-model plants for which efficient nucleic extraction procedures are lacking. In the case of grapevine, extraction of high-quality DNA is typically limited by the high polyphenolic and polysaccharide contents of the different tissues. Here, we propose an adaptation of the method of Reid et al. (2006) that allows the simultaneous and efficient extraction of DNA and RNA from grapevine vegetative and berry tissues from in vitro grown grapevine plants and cells and from other plants. The protocol allows the extraction of high-quality RNA and DNA for standard molecular biology methods as well as for Next Generation Sequencing (NGS). It also works with a limited amount of plant material, such as young developing buds, and provides the means to analyse “omics” data from a single plant sample.< Réduire

Mots clés en anglais

Vitis

Nucleic acids

DNA

RNA

Extraction protocol

Grapevine

Tomato

Rapeseed

URI

https://oskar-bordeaux.fr/handle/20.500.12278/171775

DOI

http://dx.doi.org/10.20870/oeno-one.2022.56.2.5000

Lien vers les données de la recherche

https://oeno-one.eu/article/download/5000/20181

Unités de recherche

Ecophysiologie et Génomique Fonctionnelle de la Vigne (EGFV) - UMR 1287

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One prep to catch them all: “2 in 1”, an efficient method for the simultaneous extraction of DNA and RNA from Grapevine tissues