GUYON, Joris; NOVION, Marine; FULDA, Virginie; DUCINT, Dominique; MOLIMARD, Mathieu; COUZI, Lionel; KAMINSKI, Hannah; SALVO, Francesco; BOUCHET, Stephane

doi:10.1021/jasms.2c00204

Métadonnées

Afficher la notice complète

Licence d’utilisation du document

GUYON, Joris

Bordeaux population health [BPH]

NOVION, Marine

FULDA, Virginie
Bordeaux population health [BPH]

Langue

Article de revue

Ce document a été publié dans

Journal of The American Society for Mass Spectrometry. 2022-10-05, vol. 33, n° 10, p. 1975-1981

Résumé en anglais

Nirmatrelvir/ritonavir association has been authorized for conditional use in the treatment of COVID-19, especially in solid-organ transplant recipients who did not respond to vaccine and are still at high risk of severe disease. This combination remains at risk of drug interactions with immunosuppressants, so monitoring drug levels seems necessary. After a simple protein precipitation of plasma sample, analytes were analyzed using an ultrahigh performance liquid chromatography system coupled with tandem mass spectrometry in a positive ionization mode. Validation procedures were based on the guidelines on bioanalytical methods issued by the European Medicine Agency. The analysis time was 4 min per run. The calibration curves were linear over the range from 10 to 1000 ng/mL for ritonavir and 40 to 4000 ng/mL for nirmatrelvir, with coefficients of correlation above 0.99 for all analytes. Intra-/interday imprecisions were below 10%. The analytical method also meets criteria of matrix effect, carryover, dilution integrity, and stability. In the context of a SARS-CoV-2 infection in a renal transplant recipient, we present a case of tacrolimus overdose with serious adverse events despite discontinuation of nirmatrelvir and ritonavir. The patient had still effective concentrations of nirmatrelvir and tacrolimus 4 days after drug discontinuation. This method was successfully applied for therapeutic drug monitoring in clinical practice.< Réduire

Mots clés en anglais

Nirmatrelvir

Ritonavir

Liquid chromatography

Mass spectrometry

COVID-19

Métadonnées

Partager cette publication !

Licence d’utilisation du document

A UPLC-MS/MS Method for Plasma Biological Monitoring of Nirmatrelvir and Ritonavir in the Context of SARS-CoV-2 Infection and Application to a Case

Langue

Ce document a été publié dans

Résumé en anglais

Mots clés en anglais

URI

DOI

Unités de recherche