GAILLARD, Melanie; PARLANTI, Edith; SOURZAC, Mahaut; COUILLAUD, Franck; GENEVOIS, Coralie; BOUTRY, Sebastien; RIGAUD, Christian; DAVERAT, Francoise

doi:10.1038/s41598-022-18440-0

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SCIENTIFIC REPORTS. 2022-09-26, vol. 12, n° 1

Résumé en anglais

Alizarin detection in fish fins is extensively employed because it is easy to use. However, in eels, the eelGFP fluorescent protein may impede the detection of the fluorescent markers in the eel tissues. The study tests the effectiveness of three of the most up-to-date alizarin-detecting technologies on the living body and fins of European glass eels (Anguilla anguilla L.). The findings demonstrated that the control group had a high autofluorescence at alizarin and eelGFP maxima bands. With fluorescence reflectance imaging (FRI), the eel living body autofluorescence impeded the detection of the marked eels. In contrast with experimental excitation-emission-matrix (EEM) fluorescence analyses, 99% of the marked eels were correctly assigned to their group from fluorescence analyses of their fin cellular contents. With epifluorometry (EPI), 100% of the marked eels were detected with the caudal fin tips when excited at 450–490 nm wavelengths due to a weaker autofluorescence signal. EEM and FRI assays unveiled an average fluorescence quenching 60% and 44% of the marked group respectively, in the alizarin and eelGFP maxima bands. The fluorescence quenching observed is discussed. Results will benefit experimental design by examining autofluorescence effects on mark detection and the development of non-invasive detection methods in this critically endangered species.< Réduire

Mots clés en anglais

Ecology

Imaging

Marine biology

Microscopy

Optical spectroscopy

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https://oskar-bordeaux.fr/handle/20.500.12278/170392

DOI

http://dx.doi.org/10.1038/s41598-022-18440-0

Unités de recherche

EPOC : Environnements et Paléoenvironnements Océaniques et Continentaux - UMR 5805

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New insights into detecting alizarin from autofluorescence in marked glass eels

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Résumé en anglais

Mots clés en anglais

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