GOMEZ, Rodrigo Enrique; CHAMBAUD, Clement; LUPETTE, Josselin; CASTETS, Julie; PASCAL, Stephanie; BROCARD, Lysiane; NOACK, Lise; JAILLAIS, Yvon; JOUBES, Jerome; BERNARD, Amelie

doi:10.1038/s41467-022-32109-2

Laboratoire de biogenèse membranaire [LBM]

CHAMBAUD, Clement
Laboratoire de biogenèse membranaire [LBM]

LUPETTE, Josselin
Laboratoire de biogenèse membranaire [LBM]

Langue

Article de revue

Ce document a été publié dans

Nature Communications. 2022-07-28, vol. 13, n° 1, p. 4385

Résumé en anglais

Autophagy is an intracellular degradation mechanism critical for plant acclimation to environmental stresses. Central to autophagy is the formation of specialized vesicles, the autophagosomes, which target and deliver cargo to the lytic vacuole. How autophagosomes form in plant cells remains poorly understood. Here, we uncover the importance of the lipid phosphatidylinositol-4-phosphate in autophagy using pharmacological and genetical approaches. Combining biochemical and live-microscopy analyses, we show that PI4K activity is required for early stages of autophagosome formation. Further, our results show that the plasma membrane-localized PI4Kα1 is involved in autophagy and that a substantial portion of autophagy structures are found in proximity to the PI4P-enriched plasma membrane. Together, our study unravels critical insights into the molecular determinants of autophagy, proposing a model whereby the plasma membrane provides PI4P to support the proper assembly and expansion of the phagophore thus governing autophagosome formation in Arabidopsis.< Réduire

Mots clés en anglais

Arabidopsis

Autophagosomes

Autophagy

Autophagy-Related Proteins

Phosphatidylinositol Phosphates

URI

https://oskar-bordeaux.fr/handle/20.500.12278/170270

DOI

http://dx.doi.org/10.1038/s41467-022-32109-2

Project ANR

Développment d'une infrastructure française distribuée coordonnée - ANR-10-INBS-0004

Unités de recherche

Laboratoire de Biogenèse Membranaire (LBM) - UMR 5200

Voir/Ouvrir

Métadonnées

Partager cette publication !

Licence d’utilisation du document

Phosphatidylinositol-4-phosphate controls autophagosome formation in Arabidopsis thaliana