ELIAUTOUT, Rémi; DUBRANA, Marie-Pierre; VINCENT-MONÉGAT, Carole; VALLIER, Agnès; BRAQUART-VARNIER, Christine; POIRIÉ, Marylène; SAILLARD, Colette; HEDDI, Abdelaziz; ARRICAU-BOUVERY, Nathalie

doi:10.1016/j.dci.2015.08.007

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ELIAUTOUT, Rémi
Biologie du fruit et pathologie [BFP]

DUBRANA, Marie-Pierre
Biologie du fruit et pathologie [BFP]

VINCENT-MONÉGAT, Carole
Biologie Fonctionnelle, Insectes et Interactions [BF2I]

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Article de revue

Ce document a été publié dans

Developmental and Comparative Immunology. 2015-08-13, vol. 54, n° 1, p. 7-19

Elsevier

Résumé en anglais

Spiroplasma citri is a cell wall-less bacterium that infects plants. It is transmitted by the leafhopper Circulifer haematoceps, which hosts this bacterium in the haemocel and insect tissues. Bacterial factors involved in spiroplasma colonization of the insect host have been identified, but the immune response of the leafhopper to S. citri infection remains unknown. In this study, we showed that C. haematoceps activates both humoral and cellular immune responses when challenged with bacteria. When infected by S. citri, C. haematoceps displayed a specific immune response, evidenced by activation of phagocytosis and upregulation of a gene encoding the protein hexamerin. S. citri infection also resulted in decreased phenoloxidase-like activity. Inhibition of hexamerin by RNA interference resulted in a significant reduction in phenoloxidase-like activity and increased mortality of infected leafhoppers. Therefore, the gene hexamerin is involved in S. citri control by interfering with insect phenoloxidase activity.< Réduire

Mots clés

Immune cells

RNAi

Mots clés en anglais

Hemipteran innate immunity

Hexamerin

Phenoloxidase activity