Immune response and survival of Circulifer haematoceps to Spiroplasma citri infection requires expression of the gene hexamerin.
BRAQUART-VARNIER, Christine
Écologie, Évolution, Symbiose [Équipe du laboratoire EBI Poitiers] [EES]
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Écologie, Évolution, Symbiose [Équipe du laboratoire EBI Poitiers] [EES]
Language
en
Article de revue
This item was published in
Developmental and Comparative Immunology. 2015-08-13, vol. 54, n° 1, p. 7-19
Elsevier
English Abstract
<em>Spiroplasma citri</em> is a cell wall-less bacterium that infects plants. It is transmitted by the leafhopper <em>Circulifer haematoceps</em>, which hosts this bacterium in the haemocel and insect tissues. Bacterial ...Read more >
<em>Spiroplasma citri</em> is a cell wall-less bacterium that infects plants. It is transmitted by the leafhopper <em>Circulifer haematoceps</em>, which hosts this bacterium in the haemocel and insect tissues. Bacterial factors involved in spiroplasma colonization of the insect host have been identified, but the immune response of the leafhopper to <em>S. citri</em> infection remains unknown. In this study, we showed that <em>C. haematoceps</em> activates both humoral and cellular immune responses when challenged with bacteria. When infected by <em>S. citri</em>, <em>C. haematoceps</em> displayed a specific immune response, evidenced by activation of phagocytosis and upregulation of a gene encoding the protein hexamerin. S. citri infection also resulted in decreased phenoloxidase-like activity. Inhibition of hexamerin by RNA interference resulted in a significant reduction in phenoloxidase-like activity and increased mortality of infected leafhoppers. Therefore, the gene hexamerin is involved in S. citri control by interfering with insect phenoloxidase activity.Read less <
Keywords
Immune cells
RNAi
English Keywords
Hemipteran innate immunity
Hexamerin
Phenoloxidase activity
Origin
Hal importedCollections