COLOMBIÉ, Sophie; BEAUVOIT, Bertrand; NAZARET, Christine; BÉNARD, Camille; VERCAMBRE, Gilles; LE GALL, Sophie; BIAIS, Benoit; CABASSON, Cécile; MAUCOURT, Mickaël; BERNILLON, Stéphane; MOING, Annick; DIEUAIDE-NOUBHANI, Martine; MAZAT, Jean-Pierre; GIBON, Yves

doi:10.1111/nph.14301

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COLOMBIÉ, Sophie
Biologie du fruit et pathologie [BFP]

BEAUVOIT, Bertrand
Biologie du fruit et pathologie [BFP]

NAZARET, Christine
Institut de Mathématiques de Bordeaux [IMB]

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Article de revue

Ce document a été publié dans

New Phytologist. 2016, vol. 213, n° 4, p. 1726-1739

Wiley

Résumé en anglais

Tomato is a model organism to study the development of fleshy fruit including ripening initiation. Unfortunately, few studies deal with the brief phase of accelerated ripening associated with the respiration climacteric because of practical problems involved in measuring fruit respiration. Because constraint-based modelling allows predicting accurate metabolic fluxes, we investigated the respiration and energy dissipation of fruit pericarp at the breaker stage using a detailed stoichiometric model of the respiratory pathway, including alternative oxidase and uncoupling proteins. Assuming steady-state, a metabolic dataset was transformed into constraints to solve the model on a daily basis throughout tomato fruit development. We detected a peak of CO2 released and an excess of energy dissipated at 40 d post anthesis (DPA) just before the onset of ripening coinciding with the respiration climacteric. We demonstrated the unbalanced carbon allocation with the sharp slowdown of accumulation (for syntheses and storage) and the beginning of the degradation of starch and cell wall polysaccharides. Experiments with fruits harvested from plants cultivated under stress conditions confirmed the concept. We conclude that modelling with an accurate metabolic dataset is an efficient tool to bypass the difficulty of measuring fruit respiration and to elucidate the underlying mechanisms of ripening.< Réduire

Mots clés en anglais

central metabolism

constraint-based model

flux balance analysis (FBA)

environmental stress

carbon allocation

metabolic flux modelling

respiration climacteric

tomato fruit development

DOI

http://dx.doi.org/10.1111/nph.14301

Project ANR

Développement d'une infrastructure française distribuée pour la métabolomique dédiée à l'innovation
Centre français de phénomique végétale

Origine

Importé de hal

Unités de recherche

Biologie du Fruit & Pathologie (BFP) - UMR 1332