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Respiration climacteric in tomato fruits elucidated by constraint-based modelling.

hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorCOLOMBIÉ, Sophie
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBEAUVOIT, Bertrand
hal.structure.identifierInstitut de Mathématiques de Bordeaux [IMB]
dc.contributor.authorNAZARET, Christine
hal.structure.identifierUNIROUEN - UFR Santé [UNIROUEN UFR Santé]
dc.contributor.authorBÉNARD, Camille
hal.structure.identifierUnité de recherche Plantes et Systèmes de Culture Horticoles [PSH]
dc.contributor.authorVERCAMBRE, Gilles
hal.structure.identifierInstitut de Recherche en Horticulture et Semences [IRHS]
dc.contributor.authorLE GALL, Sophie
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBIAIS, Benoit
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorCABASSON, Cécile
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorMAUCOURT, Mickaël
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBERNILLON, Stéphane
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorMOING, Annick
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorDIEUAIDE-NOUBHANI, Martine
hal.structure.identifierInstitut de biochimie et génétique cellulaires [IBGC]
dc.contributor.authorMAZAT, Jean-Pierre
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorGIBON, Yves
dc.date.issued2016
dc.identifier.issn0028-646X
dc.description.abstractEnTomato is a model organism to study the development of fleshy fruit including ripening initiation. Unfortunately, few studies deal with the brief phase of accelerated ripening associated with the respiration climacteric because of practical problems involved in measuring fruit respiration. Because constraint-based modelling allows predicting accurate metabolic fluxes, we investigated the respiration and energy dissipation of fruit pericarp at the breaker stage using a detailed stoichiometric model of the respiratory pathway, including alternative oxidase and uncoupling proteins. Assuming steady-state, a metabolic dataset was transformed into constraints to solve the model on a daily basis throughout tomato fruit development. We detected a peak of CO2 released and an excess of energy dissipated at 40 d post anthesis (DPA) just before the onset of ripening coinciding with the respiration climacteric. We demonstrated the unbalanced carbon allocation with the sharp slowdown of accumulation (for syntheses and storage) and the beginning of the degradation of starch and cell wall polysaccharides. Experiments with fruits harvested from plants cultivated under stress conditions confirmed the concept. We conclude that modelling with an accurate metabolic dataset is an efficient tool to bypass the difficulty of measuring fruit respiration and to elucidate the underlying mechanisms of ripening.
dc.description.sponsorshipDéveloppement d'une infrastructure française distribuée pour la métabolomique dédiée à l'innovation - ANR-11-INBS-0010
dc.description.sponsorshipCentre français de phénomique végétale - ANR-11-INBS-0012
dc.language.isoen
dc.publisherWiley
dc.subject.encentral metabolism
dc.subject.enconstraint-based model
dc.subject.enflux balance analysis (FBA)
dc.subject.enenvironmental stress
dc.subject.encarbon allocation
dc.subject.enmetabolic flux modelling
dc.subject.enrespiration climacteric
dc.subject.entomato fruit development
dc.title.enRespiration climacteric in tomato fruits elucidated by constraint-based modelling.
dc.typeArticle de revue
dc.identifier.doi10.1111/nph.14301
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale/Botanique
dc.subject.halMathématiques [math]
bordeaux.journalNew Phytologist
bordeaux.page1726-1739
bordeaux.volume213
bordeaux.issue4
bordeaux.peerReviewedoui
hal.identifierhal-01445653
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01445653v1
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