ROUDET, Genevieve; ANNE, Agnès; BARRA, Amandine; CHOVIN, Arnaud; DEMAILLE, Christophe; MICHON, Thierry

doi:10.1094/MPMI-04-17-0091-R

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ROUDET, Genevieve
Biologie du fruit et pathologie [BFP]

ANNE, Agnès
Laboratoire d'Electrochimie Moléculaire [LEM (UMR_7591)]

BARRA, Amandine
Biologie du fruit et pathologie [BFP]

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Article de revue

Ce document a été publié dans

Molecular Plant-Microbe Interactions. 2017, vol. 30, n° 9, p. 754-762

American Phytopathological Society

Résumé en anglais

The VPg of potyviruses is a protein covalently linked to the 5' end of viral RNA. It interacts with eIF4E, a component of the cellular translation initiation complex. It has been suggested that the 5' RNA linked VPg could mimic the cellular mRNA cap, promoting synthesis of viral proteins. We report here evidences for evidence for recruitment of the plant eIF4E by Lettuce mosaic virus (LMV, potyvirus) particles via the 5' RNA linked VPg. Analysis of the viral population was performed by ELISA-based tests, either with crude extracts of LMV-infected tissues or purified viral particles. In both cases, LMV-VPg and LMV-eIF4E subpopulations could be detected. After reaching a maximum within the first two weeks post inoculation, these populations decreased and very few labeled particles were found after 3 weeks post-inoculation. The central domain of VPg (CD-VPg) was found to be exposed at the surface of the particles. Using a purified recombinant lettuce eIF4E and CD-VPg specific antibodies, we demonstrate that the plant factor binds to the VPg via its central domain. Moreover, the plant eIF4E factor could be imaged at one end of the particles purified from LMV plant extracts, by immunoredox atomic force microscopy coupled to scanning electrochemical microscopy (AFM-SECM). We discuss the biological significance of these results.< Réduire

Mots clés

eif4e

facteur initiation eif4e

virus vpg

virus phytopathogène

potyvirus

lettuce mosaic potyvirus

lmv

virologie végétale

pathologie végétale

santé des plantes

Mots clés en anglais

virus genome linked

phytopathogenic virus

plant health

DOI

http://dx.doi.org/10.1094/MPMI-04-17-0091-R

Project ANR

Imagerie électrochimique fonctionnelle de systèmes enzymatiques multi-composants organisés sur des virus nano-gabarits - ANR-14-CE09-0009

Origine

Importé de hal

Unités de recherche

Biologie du Fruit & Pathologie (BFP) - UMR 1332