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The potyvirus particle recruits the plant translation initiation factor eIF4E by means of the VPg covalently linked to the viral RNA.

hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorROUDET, Genevieve
hal.structure.identifierLaboratoire d'Electrochimie Moléculaire [LEM (UMR_7591)]
dc.contributor.authorANNE, Agnès
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBARRA, Amandine
hal.structure.identifierUniversité Paris Diderot - Paris 7 [UPD7]
dc.contributor.authorCHOVIN, Arnaud
hal.structure.identifierUniversité Paris Diderot - Paris 7 [UPD7]
dc.contributor.authorDEMAILLE, Christophe
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorMICHON, Thierry
dc.date.issued2017
dc.identifier.issn0894-0282
dc.description.abstractEnThe VPg of potyviruses is a protein covalently linked to the 5' end of viral RNA. It interacts with eIF4E, a component of the cellular translation initiation complex. It has been suggested that the 5' RNA linked VPg could mimic the cellular mRNA cap, promoting synthesis of viral proteins. We report here evidences for evidence for recruitment of the plant eIF4E by Lettuce mosaic virus (LMV, potyvirus) particles via the 5' RNA linked VPg. Analysis of the viral population was performed by ELISA-based tests, either with crude extracts of LMV-infected tissues or purified viral particles. In both cases, LMV-VPg and LMV-eIF4E subpopulations could be detected. After reaching a maximum within the first two weeks post inoculation, these populations decreased and very few labeled particles were found after 3 weeks post-inoculation. The central domain of VPg (CD-VPg) was found to be exposed at the surface of the particles. Using a purified recombinant lettuce eIF4E and CD-VPg specific antibodies, we demonstrate that the plant factor binds to the VPg via its central domain. Moreover, the plant eIF4E factor could be imaged at one end of the particles purified from LMV plant extracts, by immunoredox atomic force microscopy coupled to scanning electrochemical microscopy (AFM-SECM). We discuss the biological significance of these results.
dc.description.sponsorshipImagerie électrochimique fonctionnelle de systèmes enzymatiques multi-composants organisés sur des virus nano-gabarits - ANR-14-CE09-0009
dc.language.isoen
dc.publisherAmerican Phytopathological Society
dc.subjecteif4e
dc.subjectfacteur initiation eif4e
dc.subjectvirus vpg
dc.subjectvirus phytopathogène
dc.subjectpotyvirus
dc.subjectlettuce mosaic potyvirus
dc.subjectlmv
dc.subjectvirologie végétale
dc.subjectpathologie végétale
dc.subjectsanté des plantes
dc.subject.envirus genome linked
dc.subject.enphytopathogenic virus
dc.subject.enplant health
dc.title.enThe potyvirus particle recruits the plant translation initiation factor eIF4E by means of the VPg covalently linked to the viral RNA.
dc.typeArticle de revue
dc.identifier.doi10.1094/MPMI-04-17-0091-R
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale/Phytopathologie et phytopharmacie
dc.subject.halSciences du Vivant [q-bio]/Microbiologie et Parasitologie/Virologie
bordeaux.journalMolecular Plant-Microbe Interactions
bordeaux.page754-762
bordeaux.volume30
bordeaux.issue9
bordeaux.peerReviewedoui
hal.identifierhal-01602674
hal.version1
hal.popularnon
hal.audienceNon spécifiée
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01602674v1
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