HILY, Jean-Michel; DEMANÈCHE, Sandrine; POULICARD, Nils; TANNIÈRES, Mélanie; DJENNANE, Samia; BEUVE, Monique; VIGNE, Emmanuelle; DEMANGEAT, Gérard; KOMAR, Véronique; GERTZ, Claude; MARMONIER, Aurélie; HEMMER, Caroline; VIGNERON, Sophie; MARAIS, Armelle; CANDRESSE, Thierry; SIMONET, Pascal; LEMAIRE, Olivier

doi:10.1111/pbi.12761

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http://creativecommons.org/licenses/by-sa/

HILY, Jean-Michel
Santé de la vigne et qualité du vin [SVQV]

DEMANÈCHE, Sandrine
Ampère, Département Bioingénierie [BioIng]

POULICARD, Nils
UMR - Interactions Plantes Microorganismes Environnement [UMR IPME]

Langue

Article de revue

Ce document a été publié dans

Plant Biotechnology Journal. 2017-08-09, vol. 16, n° 1, p. 208-220

Wiley

Résumé en anglais

For some crops, the only possible approach to gain a specific trait requires genome modification. The development of virus-resistant transgenic plants based on the pathogen-derived resistance strategy has been a success story for over three decades. However, potential risks associated with the technology, such as horizontal gene transfer (HGT) of any part of the transgene to an existing gene pool, have been raised. Here, we report no evidence of any undesirable impacts of genetically modified (GM) grapevine rootstock on its biotic environment. Using state of the art metagenomics, we analysed two compartments in depth, the targeted Grapevine fanleaf virus (GFLV) populations and nontargeted root-associated microbiota. Our results reveal no statistically significant differences in the genetic diversity of bacteria that can be linked to the GM trait. In addition, no novel virus or bacteria recombinants of biosafety concern can be associated with transgenic grapevine rootstocks cultivated in commercial vineyard soil under greenhouse conditions for over 6 years.< Réduire

Mots clés

Environmental microbiology

metagenomics

Mots clés en anglais

grapevine

safety and regulatory affairs

transgenic rootstock

DOI

http://dx.doi.org/10.1111/pbi.12761

Project ANR

Analyse de l'impact de la culture de plantes génétiquement modifiées sur la microflore associée par une approche métagénomique à haut débit - ANR-11-CESA-0005

Origine

Importé de hal

Unités de recherche

Biologie du Fruit & Pathologie (BFP) - UMR 1332