GOLYAEV, Victor; CANDRESSE, Thierry; RABENSTEIN, Frank; POOGGIN, Mikhail

doi:10.1038/s41598-019-55547-3

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GOLYAEV, Victor
Biologie et Génétique des Interactions Plante-Parasite [UMR BGPI]

CANDRESSE, Thierry
Biologie du fruit et pathologie [BFP]

RABENSTEIN, Frank
Julius Kühn-Institut [JKI]

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Ce document a été publié dans

Scientific Reports. 2019, vol. 9

Nature Publishing Group

Résumé en anglais

In plants, RNA interference (RNAi) generates small interfering (si)RNAs from entire genomes of viruses, satellites and viroids. Therefore, deep small (s)RNA sequencing is a universal approach for virome reconstruction and RNAi characterization. We tested this approach on dried barley leaves from field surveys. Illumina sequencing of sRNAs from 2 plant samples identified in both plants Hordeum vulgare endornavirus (HvEV) and barley yellow mosaic bymovirus (BaYMV) and, additionally in one plant, a novel strain of Japanese soil-borne wheat mosaic furovirus (JSBWMV). De novo and reference-based sRNA assembly yielded complete or near-complete genomic RNAs of these viruses. While plant sRNAs showed broad size distribution, viral sRNAs were predominantly 21 and 22 nucleotides long with 5'-terminal uridine or adenine, and were derived from both genomic strands. These bona fide siRNAs are presumably processed from double-stranded RNA precursors by Dicer-like (DCL) 4 and DCL2, respectively, and associated with Argonaute 1 and 2 proteins. For BaYMV (but not HvEV, or JSBWMV), 24-nucleotide sRNAs represented the third most abundant class, suggesting DCL3 contribution to antibymovirus defence. Thus, viral siRNAs are well preserved in dried leaf tissues and not contaminated by non-RNAi degradation products, enabling both complete virome reconstruction and inference of RNAi components mediating antiviral defense.< Réduire

DOI

http://dx.doi.org/10.1038/s41598-019-55547-3

Projet Européen

Diversification for Tobacco Growing Farms by the alternative crop Stevia rebaudiana Bertoni

Origine

Importé de hal

Unités de recherche

Biologie du Fruit & Pathologie (BFP) - UMR 1332