Plant virome reconstruction and antiviral RNAi characterization by deep sequencing of small RNAs from dried leaves
| hal.structure.identifier | Biologie et Génétique des Interactions Plante-Parasite [UMR BGPI] | |
| dc.contributor.author | GOLYAEV, Victor | |
| hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
| dc.contributor.author | CANDRESSE, Thierry | |
| hal.structure.identifier | Julius Kühn-Institut [JKI] | |
| dc.contributor.author | RABENSTEIN, Frank | |
| hal.structure.identifier | Biologie et Génétique des Interactions Plante-Parasite [UMR BGPI] | |
| dc.contributor.author | POOGGIN, Mikhail | |
| dc.date.issued | 2019 | |
| dc.identifier.issn | 2045-2322 | |
| dc.description.abstractEn | In plants, RNA interference (RNAi) generates small interfering (si)RNAs from entire genomes of viruses, satellites and viroids. Therefore, deep small (s)RNA sequencing is a universal approach for virome reconstruction and RNAi characterization. We tested this approach on dried barley leaves from field surveys. Illumina sequencing of sRNAs from 2 plant samples identified in both plants Hordeum vulgare endornavirus (HvEV) and barley yellow mosaic bymovirus (BaYMV) and, additionally in one plant, a novel strain of Japanese soil-borne wheat mosaic furovirus (JSBWMV). De novo and reference-based sRNA assembly yielded complete or near-complete genomic RNAs of these viruses. While plant sRNAs showed broad size distribution, viral sRNAs were predominantly 21 and 22 nucleotides long with 5'-terminal uridine or adenine, and were derived from both genomic strands. These bona fide siRNAs are presumably processed from double-stranded RNA precursors by Dicer-like (DCL) 4 and DCL2, respectively, and associated with Argonaute 1 and 2 proteins. For BaYMV (but not HvEV, or JSBWMV), 24-nucleotide sRNAs represented the third most abundant class, suggesting DCL3 contribution to antibymovirus defence. Thus, viral siRNAs are well preserved in dried leaf tissues and not contaminated by non-RNAi degradation products, enabling both complete virome reconstruction and inference of RNAi components mediating antiviral defense. | |
| dc.language.iso | en | |
| dc.publisher | Nature Publishing Group | |
| dc.rights.uri | http://creativecommons.org/licenses/by/ | |
| dc.title.en | Plant virome reconstruction and antiviral RNAi characterization by deep sequencing of small RNAs from dried leaves | |
| dc.type | Article de revue | |
| dc.identifier.doi | 10.1038/s41598-019-55547-3 | |
| dc.subject.hal | Sciences du Vivant [q-bio]/Microbiologie et Parasitologie/Virologie | |
| dc.description.sponsorshipEurope | Diversification for Tobacco Growing Farms by the alternative crop Stevia rebaudiana Bertoni | |
| bordeaux.journal | Scientific Reports | |
| bordeaux.volume | 9 | |
| bordeaux.peerReviewed | oui | |
| hal.identifier | hal-02628261 | |
| hal.version | 1 | |
| hal.popular | non | |
| hal.audience | Internationale | |
| hal.origin.link | https://hal.archives-ouvertes.fr//hal-02628261v1 | |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Scientific%20Reports&rft.date=2019&rft.volume=9&rft.eissn=2045-2322&rft.issn=2045-2322&rft.au=GOLYAEV,%20Victor&CANDRESSE,%20Thierry&RABENSTEIN,%20Frank&POOGGIN,%20Mikhail&rft.genre=article |
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