SVANELLA-DUMAS, Laurence; VERDIN, Eric; FAURE, Chantal; GERMAN-RETANA, Sylvie; GOGNALONS, Patrick; DANET, Jean-Luc; MARAIS, Armelle; CANDRESSE, Thierry

doi:10.1094/MPMI-10-13-0320-R

Métadonnées

Afficher la notice complète

Licence d’utilisation du document

http://creativecommons.org/licenses/by-sa/

SVANELLA-DUMAS, Laurence
Biologie du fruit et pathologie [BFP]

VERDIN, Eric
Unité de Pathologie Végétale [PV]

FAURE, Chantal
Biologie du fruit et pathologie [BFP]

Langue

Article de revue

Ce document a été publié dans

Molecular Plant-Microbe Interactions. 2014, vol. 27, n° 5, p. 491-497

American Phytopathological Society

Résumé en anglais

An isolate of Lettuce mosaic virus (LMV, a Potyvirus) infecting Madagascar periwinckle (Catharanthus roseus) was identified and characterized by Illumina deep sequencing. LMV-Cr has no close affinities to previously sequenced LMV isolates and represents a novel, divergent LMV clade. Inoculation experiments with other representative LMV isolates showed that they are unable to infect C. roseus, which was not known to be a host for LMV. However, three C. roseus variants of one of these isolates, LMV-AF199, could be selected and partially or completely sequenced. These variants are characterized by the accumulation of mutations affecting the C-terminal part of the CI helicase and the central part of the VPg. In particular, a Serine to Proline mutation at amino acid 143 of the VPg was observed in all three independently selected variants and is also present in the LMV-Cr isolate, making it a prime candidate as a host range determinant. Other mutations at VPg positions 65 and 144 could also contribute to the ability to infect C. roseus. Inoculation experiments involving a recombinant LMV expressing a permissive lettuce translation initiation factor 4E (eIF4E) suggest that eIF4E does not contribute to the interaction of most LMV isolates with C. roseus.< Réduire

Mots clés

evolution

Mots clés en anglais

Potyvirus

VPg

host change

genome