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Adaptation of Lettuce mosaic virus to <em>Catharanthus roseus</em> involves mutations in the central domain of the VPg.

hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorSVANELLA-DUMAS, Laurence
hal.structure.identifierUnité de Pathologie Végétale [PV]
dc.contributor.authorVERDIN, Eric
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorFAURE, Chantal
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorGERMAN-RETANA, Sylvie
hal.structure.identifierUnité de Pathologie Végétale [PV]
dc.contributor.authorGOGNALONS, Patrick
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorDANET, Jean-Luc
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorMARAIS, Armelle
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorCANDRESSE, Thierry
dc.date.issued2014
dc.identifier.issn0894-0282
dc.description.abstractEnAn isolate of Lettuce mosaic virus (LMV, a Potyvirus) infecting Madagascar periwinckle (Catharanthus roseus) was identified and characterized by Illumina deep sequencing. LMV-Cr has no close affinities to previously sequenced LMV isolates and represents a novel, divergent LMV clade. Inoculation experiments with other representative LMV isolates showed that they are unable to infect C. roseus, which was not known to be a host for LMV. However, three C. roseus variants of one of these isolates, LMV-AF199, could be selected and partially or completely sequenced. These variants are characterized by the accumulation of mutations affecting the C-terminal part of the CI helicase and the central part of the VPg. In particular, a Serine to Proline mutation at amino acid 143 of the VPg was observed in all three independently selected variants and is also present in the LMV-Cr isolate, making it a prime candidate as a host range determinant. Other mutations at VPg positions 65 and 144 could also contribute to the ability to infect C. roseus. Inoculation experiments involving a recombinant LMV expressing a permissive lettuce translation initiation factor 4E (eIF4E) suggest that eIF4E does not contribute to the interaction of most LMV isolates with C. roseus.
dc.language.isoen
dc.publisherAmerican Phytopathological Society
dc.rights.urihttp://creativecommons.org/licenses/by-sa/
dc.subjectevolution
dc.subject.enPotyvirus
dc.subject.enVPg
dc.subject.enhost change
dc.subject.engenome
dc.title.enAdaptation of Lettuce mosaic virus to <em>Catharanthus roseus</em> involves mutations in the central domain of the VPg.
dc.typeArticle de revue
dc.identifier.doi10.1094/MPMI-10-13-0320-R
dc.subject.halSciences du Vivant [q-bio]/Sciences agricoles
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale/Phytopathologie et phytopharmacie
dc.subject.halSciences du Vivant [q-bio]/Microbiologie et Parasitologie/Virologie
bordeaux.journalMolecular Plant-Microbe Interactions
bordeaux.page491-497
bordeaux.volume27
bordeaux.issue5
bordeaux.peerReviewedoui
hal.identifierhal-02632816
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02632816v1
bordeaux.COinSctx_ver=Z39.88-2004&amp;rft_val_fmt=info:ofi/fmt:kev:mtx:journal&amp;rft.jtitle=Molecular%20Plant-Microbe%20Interactions&amp;rft.date=2014&amp;rft.volume=27&amp;rft.issue=5&amp;rft.spage=491-497&amp;rft.epage=491-497&amp;rft.eissn=0894-0282&amp;rft.issn=0894-0282&amp;rft.au=SVANELLA-DUMAS,%20Laurence&amp;VERDIN,%20Eric&amp;FAURE,%20Chantal&amp;GERMAN-RETANA,%20Sylvie&amp;GOGNALONS,%20Patrick&amp;rft.genre=article


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