A DEMETER-like DNA demethylase governs tomato fruit ripening
Langue
en
Article de revue
Ce document a été publié dans
Proceedings of the National Academy of Sciences of the United States of America. 2015, vol. 112, n° 34, p. 10804-9
National Academy of Sciences
Résumé en anglais
In plants, genomic DNA methylation which contributes to development and stress responses can be actively removed by DEMETER-like DNA demethylases (DMLs). Indeed, in Arabidopsis DMLs are important for maternal imprinting ...Lire la suite >
In plants, genomic DNA methylation which contributes to development and stress responses can be actively removed by DEMETER-like DNA demethylases (DMLs). Indeed, in Arabidopsis DMLs are important for maternal imprinting and endosperm demethylation, but only a few studies demonstrate the developmental roles of active DNA demethylation conclusively in this plant. Here, we show a direct cause and effect relationship between active DNA demethylation mainly mediated by the tomato DML, SlDML2, and fruit ripening- an important developmental process unique to plants. RNAi SlDML2 knockdown results in ripening inhibition via hypermethylation and repression of the expression of genes encoding ripening transcription factors and rate-limiting enzymes of key biochemical processes such as carotenoid synthesis. Our data demonstrate that active DNA demethylation is central to the control of ripening in tomato.< Réduire
Mots clés en anglais
fruit ripening
tomato
DNA glycosylase lyase
active DNA demethylation
epigenetic
Project ANR
Développement d'une infrastructure française distribuée pour la métabolomique dédiée à l'innovation
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