A DEMETER-like DNA demethylase governs tomato fruit ripening
Language
en
Article de revue
This item was published in
Proceedings of the National Academy of Sciences of the United States of America. 2015, vol. 112, n° 34, p. 10804-9
National Academy of Sciences
English Abstract
In plants, genomic DNA methylation which contributes to development and stress responses can be actively removed by DEMETER-like DNA demethylases (DMLs). Indeed, in Arabidopsis DMLs are important for maternal imprinting ...Read more >
In plants, genomic DNA methylation which contributes to development and stress responses can be actively removed by DEMETER-like DNA demethylases (DMLs). Indeed, in Arabidopsis DMLs are important for maternal imprinting and endosperm demethylation, but only a few studies demonstrate the developmental roles of active DNA demethylation conclusively in this plant. Here, we show a direct cause and effect relationship between active DNA demethylation mainly mediated by the tomato DML, SlDML2, and fruit ripening- an important developmental process unique to plants. RNAi SlDML2 knockdown results in ripening inhibition via hypermethylation and repression of the expression of genes encoding ripening transcription factors and rate-limiting enzymes of key biochemical processes such as carotenoid synthesis. Our data demonstrate that active DNA demethylation is central to the control of ripening in tomato.Read less <
English Keywords
fruit ripening
tomato
DNA glycosylase lyase
active DNA demethylation
epigenetic
ANR Project
Développement d'une infrastructure française distribuée pour la métabolomique dédiée à l'innovation
Origin
Hal importedCollections